Haq, M.I-ul; (2005) MUTZ-3: a potential in-vitro model for the generation of dendritic-like cells. Doctoral thesis , University of London.

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Abstract

Dendritic Cells (DCs) are key regulators in autoimmunity. DCs function as antigen presenting cells (APCs) and are present in trace amounts in virtually all organs of the human body. DCs activate and regulate naive T cell function by inspecting T cell function to identify specific T cells for the antigen presented on the DC surface as peptide-MHC molecules. Currently DCs harvested from blood and bone marrow possess individual variability in function and phenotype expression. Thus the need for standardised DC model is essential to enable more efficient and controllable studies into DC function as key immunoregulatory cells. Previous studies have shown myeloid DCs generated from MUTZ-3 myeloid leukaemic cell line precursors to be a potentially suitable model for DC function studies. In this study we aimed to establish MUTZ-3 as a model for DCs by incubating with various cytokines (TPO, IL-3, FLT3L, MCSF and SCF). The phenotype of the MUTZ-3 was analysed by flow cytometry to ensure the monocyte surface marker, CD14+, was optimally expressed. Further differentiation of these monocytes to immature DC was investigated by stimulating the MUTZ-3 with GM-CSF and IL-4. The results obtained are preliminary and require repeating, however, most have been shown to be reproducible. It was observed that cytokines can differentiate MUTZ-3 to optimise CD14+ monocytic expression. In addition, GM-CSF and IL-4 were shown to differentiate these monocytes to immature-DC. Finally, retinoic acid was used to differentiate the MUTZ-3. However, it is vital to continue investigating to establish the most efficient method for CD14+ expression is optimised.

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