Yusuf, Mohammed;
Parmar, Neha;
Bhella, Gurdeep K;
Robinson, Ian K;
(2014)
A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension.
BioTechniques
, 56
(5)
pp. 257-261.
10.2144/000114168.
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Abstract
Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simple reverse filtration step. Fluorescence microscopy was used to validate the chromosome suspension after each filtration step. This reverse filtration technique is an improvement in both procedure time and chromosome recovery compared to currently used post-purification methods. Chromosomes purified by our method could be used for many applications, such as structural studies using microfluidics and high resolution imaging or generation of chromosome paints and sequencing after flow cytometry.
| Type: | Article |
|---|---|
| Title: | A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.2144/000114168 |
| Publisher version: | https://doi.org/10.2144/000114168 |
| Language: | English |
| Additional information: | © The Author(s), 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ |
| Keywords: | chromosome, cytoplasmic debris, filtration, mitotic, nuclei, |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1433921 |
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