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Low dose of propranolol does not affect rat osteotomy healing and callus strength

Smitham, P; Goodship, A; Blunn, G; Crossfield, L; Hughes, G; Chenu, C; (2014) Low dose of propranolol does not affect rat osteotomy healing and callus strength. Journal of Orthopaedic Research , 32 (7) 887 - 893. 10.1002/jor.22619. Green open access

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Abstract

Experimental studies suggest that the β-blocker propranolol stimulates bone formation but little work has investigated its effect on fracture healing. In this study, we examined if a low dose of propranolol, previously shown to be preventive against bone loss in rats, improves bone repair. Female Wistar rats were injected with saline or propranolol (0.1-mg/kg/day) (n-=-20/group), 5 days a week for 8 weeks. Three weeks after the beginning of treatment, all rats underwent a mid-diaphyseal transverse osteotomy in the left femur. Radiographic analysis of ostetomy healing was performed 2 and 5 weeks after osteotomy. Rats were sacrificed at 5 weeks and femora collected for measurements of fracture strength by torsional testing, callus volume, and mineral content by micro-CT analysis and histology of fracture callus. Eighty nine percent of osteotomies achieved apparent radiological union by 5 weeks in both groups. Propranolol treatment did not significantly alter the torsional strength of the fractured femur compared with controls. The volume and mineralization of fracture callus at 5 weeks were not significantly different in both groups. Histology showed that endochondral ossification was not affected by propranolol. Altogether, our results demonstrate that propranolol using the regimen described does not significantly improve or inhibit rat osteotomy healing and mechanical strength. © 2014 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc.

Type: Article
Title: Low dose of propranolol does not affect rat osteotomy healing and callus strength
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/jor.22619
Publisher version: http://dx.doi.org/10.1002/jor.22619
Additional information: © 2014 The Authors. Journal of Orthopaedic Research Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Keywords: propranolol; bone architecture; fracture healing; micro-CT; mechanical testing;
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
URI: https://discovery.ucl.ac.uk/id/eprint/1431620
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