Pyne, A;
Thompson, R;
Leung, C;
Roy, D;
Hoogenboom, BW;
(2014)
Single-Molecule Reconstruction of Oligonucleotide Secondary Structure by Atomic Force Microscopy.
Small
, 10
(16)
pp. 3257-3261.
10.1002/smll.201400265.
![[thumbnail of smll201400265.pdf]](https://discovery.ucl.ac.uk/style/images/fileicons/application_pdf.png) Preview |
PDF
smll201400265.pdf Download (1MB) |
Abstract
Based on soft-touch atomic force microscopy, a method is described to reconstruct the secondary structure of single extended biomolecules, without the need for crystallization. The method is tested by accurately reproducing the dimensions of the B-DNA crystal structure. Importantly, intramolecular variations in groove depth of the DNA double helix are resolved, which would be inaccessible for methods that rely on ensemble-averaging.
| Type: | Article |
|---|---|
| Title: | Single-Molecule Reconstruction of Oligonucleotide Secondary Structure by Atomic Force Microscopy. |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/smll.201400265 |
| Publisher version: | http://dx.doi.org/10.1002/smll.201400265 |
| Additional information: | © 2014 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | DNA, atomic force microscopy, biomolecules, biophysics, oligonucleotides |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Physics and Astronomy UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1427322 |
Archive Staff Only
 |
View Item |
