Moody, P;
Chudasama, V;
Nathani, RI;
Maruani, A;
Martin, S;
Smith, ME;
Caddick, S;
(2014)
A rapid, site-selective and efficient route to the dual modification of DARPins.
Chem Commun (Camb)
, 50
(38)
pp. 4898-4900.
10.1039/c4cc00053f.
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Abstract
Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.
| Type: | Article |
|---|---|
| Title: | A rapid, site-selective and efficient route to the dual modification of DARPins. |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c4cc00053f |
| Publisher version: | http://dx.doi.org/10.1039/c4cc00053f |
| Additional information: | © Royal Society of Chemistry 2014. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1426712 |
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