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Robust, microfabricated culture devices with improved control over the soluble microenvironment for the culture of embryonic stem cells.

Macown, RJ; Veraitch, FS; Szita, N; (2014) Robust, microfabricated culture devices with improved control over the soluble microenvironment for the culture of embryonic stem cells. Biotechnol J , 9 (6) pp. 805-813. 10.1002/biot.201300245. Green open access

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Abstract

The commercial use of stem cells continues to be constrained by the difficulty and high cost of developing efficient and reliable production protocols. The use of microfabricated systems combines good control over the cellular microenvironment with reduced use of resources in process optimization. Our previously reported microfabricated culture device was shown suitable for the culture of embryonic stem cells but required improvements to robustness, ease of use and dissolved gas control. In this report we describe a number of improvements to the design of the microfabricated system to significantly improve the control over shear stress and soluble factors, particularly dissolved oxygen. These control improvements are investigated by finite element modeling. Design improvements also make the system easier to use and improve the robustness. The culture device could be applied to the optimization of pluripotent stem cell growth and differentiation, as well as the development of monitoring and control strategies and improved culture systems at various scales.

Type: Article
Title: Robust, microfabricated culture devices with improved control over the soluble microenvironment for the culture of embryonic stem cells.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/biot.201300245
Publisher version: http://dx.doi.org/10.1002/biot.201300245
Additional information: © 2014 The Authors. Biotechnology Journal published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords: Cell culture, Microfluidic culture device, Microreactor, Process development, Stem cells
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1426389
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