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Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice.

Bryson, JB; Machado, CB; Crossley, M; Stevenson, D; Bros-Facer, V; Burrone, J; Greensmith, L; (2014) Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice. Science , 344 (6179) 94 - 97. 10.1126/science.1248523. Green open access

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Abstract

Damage to the central nervous system caused by traumatic injury or neurological disorders can lead to permanent loss of voluntary motor function and muscle paralysis. Here, we describe an approach that circumvents central motor circuit pathology to restore specific skeletal muscle function. We generated murine embryonic stem cell-derived motor neurons that express the light-sensitive ion channel channelrhodopsin-2, which we then engrafted into partially denervated branches of the sciatic nerve of adult mice. These engrafted motor neurons not only reinnervated lower hind-limb muscles but also enabled their function to be restored in a controllable manner using optogenetic stimulation. This synthesis of regenerative medicine and optogenetics may be a successful strategy to restore muscle function after traumatic injury or disease.

Type: Article
Title: Optical control of muscle function by transplantation of stem cell-derived motor neurons in mice.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1126/science.1248523
Publisher version: http://dx.doi.org/10.1126/science.1248523
Language: English
Additional information: This isthe author’s version of the work. It is posted here by permission of the AAAS for personal use, not for redistribution. The definitive version was published in Science 4 April 2014: Vol. 344 no. 6179 pp. 94-97 DOI: 10.1126/science.1248523
Keywords: Animals, Axons, Cell Line, Electric Stimulation, Embryonic Stem Cells, Female, Hindlimb, Isometric Contraction, Light, Mice, Mice, Inbred C57BL, Motor Neurons, Muscle Denervation, Muscle Fibers, Skeletal, Muscle, Skeletal, Nerve Regeneration, Optogenetics, Rhodopsin, Sciatic Nerve, Transfection, Transgenes
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/1426329
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