UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Therapeutic foam scaffolds incorporating biopolymer-shelled mesoporous nanospheres with growth factors

Kim, TH; Eltohamy, M; Kim, M; Perez, RA; Kim, JH; Yun, YR; Jang, JH; ... Kim, HW; + view all (2014) Therapeutic foam scaffolds incorporating biopolymer-shelled mesoporous nanospheres with growth factors. Acta Biomaterialia , 10 (6) pp. 2612-2621. 10.1016/j.actbio.2014.02.005. Green open access

[img]
Preview
PDF (Graphical abstract)
Acta_Biomaterialia_10_6_2612.pdf

Download (2MB)
[img]
Preview
Other (JPG Fig. 1)
1-s2.0-S1742706114000579-gr1.jpg

Download (51kB)
[img]
Preview
Other (JPG Fig. 2)
1-s2.0-S1742706114000579-gr2.jpg

Download (92kB)
[img] Excel Spreadsheet (Table 1. Summary of BET and ζ-potential results of the nanoparticle samples; aminated eMSN with and without BSA loading.)
table.csv

Download (278B)
[img]
Preview
PDF (JPG Fig. 3)
1-s2.0-S1742706114000579-gr3.jpg

Download (30kB)
[img]
Preview
PDF (JPG Fig. 4)
1-s2.0-S1742706114000579-gr4.jpg

Download (83kB)
[img]
Preview
PDF (JPG Fig. 5)
1-s2.0-S1742706114000579-gr5.jpg

Download (60kB)
[img]
Preview
PDF (JPG Fig. 6)
1-s2.0-S1742706114000579-gr6.jpg

Download (210kB)

Abstract

Here we report a novel therapeutic scaffolding system of engineered nanocarriers within a foam matrix for the long-term and sequential delivery of growth factors. For this, mesoporous silica nanospheres were first functionalized to have enlarged mesopore size (eMSNs, 12.2 nm) and aminated surface, which was then shelled by a biopolymer, poly(lactic acid) (PLA) or poly(ethylene glycol) (PEG) via an electrospraying. The hybrid nanocarrier was subsequently combined with collagen to produce foam scaffolds. Bovine serum albumin (BSA), used as a model protein, was effectively loaded within the enlarged nanospheres. The biopolymer shell substantially prolonged the release period of BSA (over 2-3 weeks from shelled nanospheres vs. within 1 week from bare nanospheres), and the release rate was highly dependent on the shell composition (PEG > PLA). Collagen foam scaffolding of the shelled nanocarrier further slowed down the protein release while enabling the incorporation of a rapidly releasing protein, which is effective for the sequential protein delivery. Acidic fibroblast growth factor (aFGF), loaded onto the shelled-nanocarrier scaffolds, was released over a month at a highly sustainable rate, profiling a similar release pattern to BSA. Biological activity of the aFGF released from the system, as examined by the proliferative potential of osteoblastic precursor cells, showed a significant improvement in the case with aFGF. Furthermore, in vivo implantation of the aFGF-delivering system in a rat subcutaneous tissue for 2 weeks showed a substantially enhanced invasion of fibroblasts with a homogeneous population. Taken together, it is concluded that the biopolymer encapsulation of mesoporous nanospheres effectively prolongs the release of growth factors over weeks to a month, providing a nanocarrier platform for a long-term growth factor delivery, moreover, the foam scaffolding of the nanocarrier system will be a potential therapeutic 3D matrix for cell culture and tissue engineering.

Type: Article
Title: Therapeutic foam scaffolds incorporating biopolymer-shelled mesoporous nanospheres with growth factors
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.actbio.2014.02.005
Publisher version: http://dx.doi.org/10.1016/j.actbio.2014.02.005
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits use, distribution, and reproduction in any medium, provided that that reuse is restricted to non-commercial purposes, i.e. research or educational use, and the original work is properly cited.
Keywords: Growth factors, Mesoporous nanospheres, Protein delivery, Scaffolds, Therapeutic system, Tissue regeneration
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1424270
Downloads since deposit
1,612Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item