Kim, TH;
Eltohamy, M;
Kim, M;
Perez, RA;
Kim, JH;
Yun, YR;
Jang, JH;
... Kim, HW; + view all
(2014)
Therapeutic foam scaffolds incorporating biopolymer-shelled mesoporous nanospheres with growth factors.
Acta Biomaterialia
, 10
(6)
pp. 2612-2621.
10.1016/j.actbio.2014.02.005.
Preview |
PDF (Graphical abstract)
Acta_Biomaterialia_10_6_2612.pdf Download (2MB) |
Preview |
Other (JPG Fig. 1)
1-s2.0-S1742706114000579-gr1.jpg Download (51kB) |
Preview |
Other (JPG Fig. 2)
1-s2.0-S1742706114000579-gr2.jpg Download (92kB) |
Excel Spreadsheet (Table 1. Summary of BET and ζ-potential results of the nanoparticle samples; aminated eMSN with and without BSA loading.)
table.csv Download (278B) |
|
Preview |
PDF (JPG Fig. 3)
1-s2.0-S1742706114000579-gr3.jpg Download (30kB) |
Preview |
PDF (JPG Fig. 4)
1-s2.0-S1742706114000579-gr4.jpg Download (83kB) |
Preview |
PDF (JPG Fig. 5)
1-s2.0-S1742706114000579-gr5.jpg Download (60kB) |
Preview |
PDF (JPG Fig. 6)
1-s2.0-S1742706114000579-gr6.jpg Download (210kB) |
Abstract
Here we report a novel therapeutic scaffolding system of engineered nanocarriers within a foam matrix for the long-term and sequential delivery of growth factors. For this, mesoporous silica nanospheres were first functionalized to have enlarged mesopore size (eMSNs, 12.2 nm) and aminated surface, which was then shelled by a biopolymer, poly(lactic acid) (PLA) or poly(ethylene glycol) (PEG) via an electrospraying. The hybrid nanocarrier was subsequently combined with collagen to produce foam scaffolds. Bovine serum albumin (BSA), used as a model protein, was effectively loaded within the enlarged nanospheres. The biopolymer shell substantially prolonged the release period of BSA (over 2-3 weeks from shelled nanospheres vs. within 1 week from bare nanospheres), and the release rate was highly dependent on the shell composition (PEG > PLA). Collagen foam scaffolding of the shelled nanocarrier further slowed down the protein release while enabling the incorporation of a rapidly releasing protein, which is effective for the sequential protein delivery. Acidic fibroblast growth factor (aFGF), loaded onto the shelled-nanocarrier scaffolds, was released over a month at a highly sustainable rate, profiling a similar release pattern to BSA. Biological activity of the aFGF released from the system, as examined by the proliferative potential of osteoblastic precursor cells, showed a significant improvement in the case with aFGF. Furthermore, in vivo implantation of the aFGF-delivering system in a rat subcutaneous tissue for 2 weeks showed a substantially enhanced invasion of fibroblasts with a homogeneous population. Taken together, it is concluded that the biopolymer encapsulation of mesoporous nanospheres effectively prolongs the release of growth factors over weeks to a month, providing a nanocarrier platform for a long-term growth factor delivery, moreover, the foam scaffolding of the nanocarrier system will be a potential therapeutic 3D matrix for cell culture and tissue engineering.
Type: | Article |
---|---|
Title: | Therapeutic foam scaffolds incorporating biopolymer-shelled mesoporous nanospheres with growth factors |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1016/j.actbio.2014.02.005 |
Publisher version: | http://dx.doi.org/10.1016/j.actbio.2014.02.005 |
Language: | English |
Additional information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits use, distribution, and reproduction in any medium, provided that that reuse is restricted to non-commercial purposes, i.e. research or educational use, and the original work is properly cited. |
Keywords: | Growth factors, Mesoporous nanospheres, Protein delivery, Scaffolds, Therapeutic system, Tissue regeneration |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng |
URI: | https://discovery.ucl.ac.uk/id/eprint/1424270 |
Archive Staff Only
View Item |