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Biological performance of titania containing phosphate-based glasses for bone tissue engineering applications

Abou Neel, EA; Chrzanowski, W; Knowles, JC; (2014) Biological performance of titania containing phosphate-based glasses for bone tissue engineering applications. Materials Science and Engineering: C , 35 307 - 313. 10.1016/j.msec.2013.10.029. Green open access

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Abstract

The interplay between glass chemistry, structure, degradation kinetics, and biological activity provides flexibility for the development of scaffolds with highly specific cellular response. The aim of this study was therefore to investigate the role of titania inclusion into the phosphate-based glass on its ability to stimulate osteoblast-like human osteosarcoma (HOS) cells to adhere, proliferate and differentiate. In depth morphological and biochemical characterisation was performed on HOS cells cultured on the surface of glass discs. Cell proliferation was also studied in the presence of the glass extract. Cell differentiation, through osteoblast phenotype genes, alkaline phosphatase (ALP) activity and osteocalcin production, was carried out using normal or osteogenic media. Both Thermanox® and titania free glass were used as controls. The data demonstrated that titania inclusion provides desired cytocompatible surface that supported initial cell attachment, sustained viability, and increased cell proliferation similar or significantly higher than Thermanox®. The modified glasses regulated osteoblastic cell differentiation as detected by osteoblast phenotype gene transcription and upregulated ALP and osteocalcin expression. Using osteogenic media had no significant effect on ALP activity and osteocalcin expression. Therefore, titania modified phosphate glasses may have future use as bone tissue engineering scaffolds.

Type: Article
Title: Biological performance of titania containing phosphate-based glasses for bone tissue engineering applications
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.msec.2013.10.029
Publisher version: http://dx.doi.org/10.1016/j.msec.2013.10.029
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Alkaline phosphatase and osteocalcin expression, Bone tissue engineering, Cell proliferation, Cell viability, Glass scaffolds, Phosphate glasses
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/1424269
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