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[(ma)21.9(in)18.4(te)18.6(na)16.1(nc)22.6(e)-297.1(o)0(f)-287.8(t)0(h)15.1(e)-290.7(sh)14.3(a)13.1(p)0(e)17.7(.)-293.8(Ne)19.1(ve)18.3(rt)17.8(he)15.7(le)21.9(ss)16.3(,)-293.8(i)0(t)21.3(s)-298.5(bi)17.3(ol)19(og)24.4(ic)16.2(a)13.1(l)-300.7(pr)23.2(op)14.8(e)12.7(r)0(-)]TJ
0 -1.2266 TD
[(ti)21.3(es)-216.2(ar)18.8(e)-227.5(p)0(o)21.2(o)0(r)-218.4(i)0(n)-215.6(t)12.2(er)18.4(ms)-213.9(of)-218.2(ce)19.8(ll)-215.7(ad)16.5(he)22(si)20.4(on)19.1(,)-224.2(m)0(i)17.9(g)0(r)20.2(a)0(t)19(i)0(o)19(n)0(,)-215(a)0(n)16.1(d)-224.3(vi)21(a)13.1(b)0(i)17.3(l)0(i)18.2(t)12.2(y,)]TJ
0 -1.2203 TD
[(an)16.1(d)-445.6(t)0(h)15.1(u)0(s)22.6(,)-451.8(it)-440.3(ha)16.1(s)-450.2(u)0(s)22.6(u)0(a)18.1(l)0(l)18.2(y)-453.3(b)14.6(e)0(e)19.2(n)-452.3(co)16.9(mb)23.3(in)18.4(ed)-439.2(wi)20.1(th)-446.5(ot)22.1(he)15.7(r)-449.6(n)0(a)22.4(t)0(u)17.1(r)0(a)18.8(l)]TJ
T*
[(po)21.2(ly)17.3(me)21.5(rs)-267.6(or)-262.7(sp)22.6(ec)19.8(i)18.8(c)-271.2(s)0(e)17.8(q)0(u)19.5(e)0(n)15.7(c)13.4(es)-266.8(su)22.6(ch)-268.2(a)13.1(s)-273.2(a)13.1(r)0(g)20.2(i)0(n)18.4(i)0(n)18.4(-)0(g)20.4(l)0(y)17.3(c)0(i)16.2(n)0(-)21.4(a)0(s)18.1(p)0(a)18.1(r)0(t)17.8(i)15.4(c)]TJ
T*
[(ac)20.2(id)-234.2(\()12.2(R)0(G)16(D)0(\))-229.5(t)12.2(o)-243(e)12.7(nh)12.2(a)13.1(n)0(c)16.3(e)-240.2(t)12.2(h)0(e)-230.9(c)0(e)19.8(l)0(l)-234.7(s)0(u)22.6(r)0(v)17.5(i)0(v)21(a)0(l)-230.7(r)0(a)18.8(t)0(e)18.6(.)]TJ
6.7246 0 0 5.9768 230.9102 499.6345 Tm
(16)Tj
8.9663 0 0 8.9663 72 484.6676 Tm
[(W)13.6(e)-252.8(ai)15.9(me)21.5(d)-255.9(t)12.2(o)-255.7(d)0(e)22.4(v)0(e)18.3(l)0(o)19(p)-254.3(a)-252.4(s)0(y)19.6(s)0(t)17.2(e)12.8(m)-250.5(t)0(h)15.1(a)13.1(t)-253.4(u)0(t)17.1(i)0(l)18.2(i)0(z)22.2(e)0(s)-247.8(t)12.2(he)-243.6(be)21(st)-248.4(pr)23.2(op)14.8(e)12.7(r)0(-)]TJ
-1.1128 -1.2203 TD
[(ti)21.3(es)-285.7(of)-287.8(al)15.9(g)14.6(i)0(n)18.4(a)0(t)19(e)-297.1(a)13.1(n)0(d)-284.6(c)0(o)16.9(l)0(l)18.2(a)13.1(ge)14.7(n,)-284.5(a)13.1(n)0(d)-284.6(t)0(o)-287.8(d)0(e)16.1(v)0(e)18.3(l)15.4(op)-288.7(a)-296.7(s)0(i)20.4(m)0(p)20.1(l)0(e)-288(p)0(r)23.2(o)0(c)16.9(e)12.7(ss)]TJ
T*
[(al)22.2(lo)19(wi)13.8(ng)-153.2(th)15.1(e)-164.3(e)0(x)16.9(t)12.2(ru)16.9(si)20.4(on)-157.9(of)-155(la)15.9(rg)20.2(e)-164.3(v)0(o)15.5(l)0(u)20.4(m)0(e)21.5(s)-165.7(of)-161.3(e)12.8(n)0(c)16.3(a)0(p)18.1(s)0(u)22.6(l)0(a)15.9(t)12.2(ed)-161(c)13.4(e)0(l)15.5(l)0(s)20.5(.)-167.3(A)]TJ
0 -1.2266 TD
[(no)19.1(ve)18.3(l)-167.9(s)0(t)17.2(r)0(u)16.9(c)13.4(tu)17.1(re)18.4(d)-167.4(c)0(e)19.8(l)0(l)-158.8(d)0(e)16.1(l)0(i)18.2(v)0(e)18.3(r)0(y)-156.9(s)0(y)19.6(s)0(t)17.2(e)12.7(m)-168.3(w)0(a)17.8(s)-172(de)22.4(ve)18.3(lo)19(pe)17.7(d)-167.4(b)0(a)21.4(s)0(e)17.8(d)-167.4(on)-164.2(a)]TJ
0 -1.2203 TD
[(hy)17.5(dr)21.6(og)18.1(e)12.8(l)-149(co)16.9(ns)20.6(is)20.4(ti)15(ng)-140.6(of)-136(th)15.1(e)-145.3(c)0(o)16.9(l)0(l)24.6(a)0(g)15(e)12.8(n)-148.8(i)0(n)18.4(n)0(e)15.7(r)-146.1(co)16.9(re)-133.4(an)16.1(d)-148.4(a)13.1(lg)17.3(in)18.4(a)13.1(t)0(e)-139.5(o)0(u)14.8(t)12.2(er)]TJ
T*
[(sh)20.6(el)15.5(l,)-335.2(wh)20.3(e)12.7(r)0(e)18.4(b)0(y)-337.6(c)13.4(el)15.5(l)15.4(s)-349.1(a)13.1(r)0(e)-335.7(l)0(o)19(a)13.1(de)16.1(d)-344.4(a)13.1(nd)-335.2(de)16.1(li)24.6(ve)18.3(re)18.4(d)-344.4(i)0(n)-335.7(t)12.2(he)-338.4(c)13.4(o)0(l)19(l)0(a)15.9(g)14.5(en)]TJ
0 -1.2266 TD
[(in)18.4(ne)15.7(r)-405.4(p)0(a)18.1(r)0(t)17.8(.)-401.3(Sp)19.1(ec)13.5(i)25.1(ca)13.9(l)15.4(l)0(y)17.3(,)-407.6(t)12.2(h)0(e)-401.6(c)0(o)16.9(r)0(e)18.4()13.1(sh)20.6(el)15.5(l)-408.2(h)0(y)23.8(d)0(r)15.3(o)0(g)24.4(e)0(l)-401.8(c)13.4(el)15.5(l)-408.2(d)0(e)22.4(l)0(i)18.2(v)0(e)18.3(r)0(y)]TJ
0 -1.2203 TD
[(sy)19.6(st)17.2(e)12.8(m)-256.8(wa)17.8(s)-260.6(p)0(r)23.2(o)0(d)19.5(u)0(c)18.3(e)0(d)-249.5(i)0(n)-247.2(a)-258.8(b)17.9(ro)21.8(us)-249.3(st)17.2(ru)23.2(ct)19.2(ur)16.9(e)-259.1(u)0(s)16.3(i)15.4(ng)-248.1(a)-258.7(s)0(i)20.4(m)0(p)20(l)0(e)-250(r)0(o)15.5(-)]TJ
T*
[(bo)18.1(t)12.2(i)0(c)-211.5(d)0(i)25.1(s)0(p)16.3(e)0(n)15.7(s)0(i)20.4(n)0(g)-203.8(m)0(a)15.6(c)13.4(hi)18.4(ne)15.7(,)-211.6(w)0(h)14(i)0(c)22.5(h)-218.4(we)-203.9(ha)16.1(ve)-209.3(pr)23.2(ev)18.3(io)19(us)16.3(ly)-204(us)16.3(e)12.7(d)-218(a)13.1(s)-222.6(a)]TJ
0 -1.2266 TD
[(du)20.9(al)-369.8(dr)21.6(ug)-372.5(d)16(e)0(l)15.5(i)0(v)21(e)0(r)18.4(y)-377.5(sy)19.6(st)17.2(e)12.8(m)0(.)]TJ
6.7246 0 0 5.9768 169.1716 390.0471 Tm
(17)Tj
8.9663 0 0 8.9663 179.2062 386.0219 Tm
[(De)17.7(po)21.2(si)20.4(ti)15(on)-366.6(of)-370(b)17.9(e)12.7(r)0(s)-368.7(w)0(a)17.8(s)-374.4(ma)15.6(d)16(e)]TJ
-13.0695 -1.2203 TD
[(th)15.1(ro)21.8(ug)19.5(h)-167.8(t)0(h)15.1(e)-164.3(co)16.9(nc)16.3(e)12.8(n)0(t)15.1(r)0(i)21.1(c)-170(no)19.1(z)13.1(z)0(l)22.2(e)-170.6(in)18.4(t)12.2(o)-173.5(a)13.1(n)-167.8(ad)16.5(j)14(u)0(s)16.3(t)12.2(ed)-161(C)12.5(a)]TJ
6.7246 0 0 5.9768 248.4283 379.0487 Tm
(2)Tj
/F9 1 Tf
.6576 0 TD
(+)Tj
/F3 1 Tf
8.9663 0 0 8.9663 257.5558 375.0802 Tm
[(-c)19.2(on)12.8(t)12.2(a)0(i)22.2(n)0(i)18.4(n)0(g)]TJ
-21.8077 -1.2203 TD
[(ba)21.4(th)15.1(.)-230.5(T)0(h)18.9(e)-233.8(cr)19(os)21.2(sl)20.4(in)18.4(ki)17.3(ng)-222.8(of)-224.5(a)13.1(l)0(g)17.3(i)0(n)18.4(a)13.1(te)-228(a)13.1(i)0(d)18.8(e)0(d)-224.2(t)12.2(he)-224.6(st)17.2(ru)16.9(c)13.4(t)0(u)17.1(r)0(a)18.8(l)-231.2(st)17.2(a)13.1(b)0(i)17.3(l)0(i)18.2(t)12.2(y,)]TJ
T*
[(wh)20.3(il)18.2(e)-297.1(m)0(a)21.9(i)0(n)18.4(t)0(a)19(i)0(n)18.4(i)0(n)18.4(g)-295.3(th)15.1(e)-297.1(s)0(h)20.6(a)13.1(pe)-292.1(of)-281.4(th)15.1(e)-297.1(c)0(o)16.9(l)15.4(la)15.9(ge)21(n)-300.6(c)13.4(or)15.5(e)12.7(.)-293.8(Th)18.9(e)-297.1(p)0(r)16.9(o)0(c)16.9(e)12.7(s-)]TJ
0 -1.2266 TD
[(si)20.4(ng)-469.4(t)12.2(o)0(o)19.7(l)0(s)-466.4(u)0(s)16.3(e)12.8(d)-477.2(t)12.2(o)-477(g)0(e)14.7(n)0(e)22(r)0(a)18.8(t)12.2(e)-480.4(t)12.2(he)-464.9(no)19.1(ve)18.3(l)-477.8(c)0(o)16.9(r)0(e)24.7()0(s)18.1(h)0(e)15.7(l)0(l)-462.3(s)0(t)17.2(r)0(u)16.9(c)13.4(tu)17.1(re)18.4(d)]TJ
0 -1.2203 TD
[(co)16.9(ll)18.2(a)13.1(g)0(e)21(n)0(-)15.1(a)13.1(lg)17.3(in)18.4(a)13.1(t)0(e)-272.3(h)0(y)17.5(d)0(r)21.6(o)0(g)18.1(e)0(l)-269(c)0(a)13.9(r)0(r)23.9(i)0(e)15.5(r)0(s)-267.6(a)0(r)18.8(e)-284.4(de)22.4(sc)18.4(ri)21.1(be)14.7(d,)-265.2(an)16.1(d)-281.2(t)12.2(he)-275.2(vi)21(a-)]TJ
T*
[(bi)17.3(li)24.6(ty)-491.7(an)16.1(d)-496.2(p)0(r)16.9(o)0(l)19(i)0(f)21.3(e)0(r)18.4(a)0(t)19(i)0(o)19(n)-502.9(of)-490.1(me)21.5(se)17.8(nc)16.3(hy)23.8(ma)15.6(l)-496.7(s)0(t)17.2(e)0(m)-490.6(c)13.4(el)15.5(ls)-491.7(\()-70(M)0(S)15.4(C)12.5(s\))]TJ
0 -1.2266 TD
[(wi)20.1(th)15.1(in)-152.3(th)15.1(e)-164.3(h)0(y)23.8(d)0(r)15.3(o)0(g)24.4(e)0(l)-155.2(c)0(a)20.2(r)0(r)17.6(i)0(e)15.5(r)0(s)-153.8(a)13.1(s)-165.7(w)0(e)17.4(l)0(l)-152.5(a)0(s)-152.6(t)0(h)15.1(e)12.8(ir)-156(os)14.9(t)12.2(e)0(o)16.3(g)14.6(en)15.7(ic)-154.6(po)21.2(te)18.6(nt)15.1(ia)22.3(l)]TJ
/F8 1 Tf
0 -1.2203 TD
[(in)-284.5(vi)17.6(tr)17.8(o)]TJ
/F3 1 Tf
3.2816 0 TD
[(un)20.6(de)16.1(r)-291.6(d)0(e)16.1()0(n)18.9(e)12.8(d)-293.9(b)0(i)17.3(o)0(c)16.9(h)0(e)22(m)0(i)17.9(c)13.4(al)-287.6(cu)18.3(es)-285.7(we)17.4(re)-285.1(in)18.4(ve)18.3(st)17.2(ig)23.7(at)19(ed)16.1(.)-293.8(F)0(i)17.3(-)]TJ
-3.2816 -1.2203 TD
[(na)16.1(l)15.4(l)0(y)17.3(,)-338(th)15.1(e)-335(a)0(b)15(i)0(l)24.6(i)0(t)15(y)-339.5(t)12.2(o)-337.9(de)16.1(li)18.2(ve)18.3(r)-335.8(M)0(S)15.4(C)12.5(s)]TJ
/F8 1 Tf
14.8778 0 TD
[(in)-328.8(v)13.1(i)0(v)17.6(o)]TJ
/F3 1 Tf
3.1678 0 TD
[(a)13.1(n)0(d)-328.9(t)0(h)15.1(e)12.8(ir)-333(ne)22(w)-343.1(b)14.6(on)19.1(e)]TJ
-18.0456 -1.2266 TD
[(fo)15.7(rm)20.7(a)13.1(t)0(i)15(o)0(n)-151.6(w)0(e)17.4(r)0(e)-158.7(a)13.1(dd)19.3(re)18.4(ss)16.3(e)12.8(d)-167.4(in)-158.7(a)-163.9(r)0(a)18.8(t)-164.9(c)13.4(a)0(l)15.9(v)0(a)18.7(r)0(i)21.1(u)0(m)-157(m)0(o)18.6(d)0(e)22.4(l)0(,)-158.2(w)0(h)20.3(i)0(c)22.5(h)-167.8(ma)21.9(y)]TJ
0 -1.2203 TD
[(pr)16.9(ov)21.8(id)18.8(e)-316(u)0(s)16.3(e)12.8(fu)17.1(l)-313.4(i)0(n)18.4(f)12.2(or)15.5(ma)21.9(ti)21.3(on)-309.7(on)-303.3(th)15.1(e)-316(u)0(s)22.6(e)-316(of)-306.7(t)12.2(h)0(e)-306.8(s)0(y)19.6(s)0(t)17.2(e)0(m)-307.3(i)0(n)-304.1(d)0(e)22.4(l)0(i)18.2(v)0(-)]TJ
T*
[(er)18.4(in)18.4(g)-238.4(s)0(t)17.2(e)12.8(m)-244.2(c)13.4(el)15.5(ls)-232.5(f)12.2(o)0(r)-231.1(b)0(o)18.1(n)0(e)-230.9(t)12.2(is)14.1(su)22.6(e)-240.2(e)0(n)15.7(g)0(i)23.7(n)0(e)15.7(e)0(r)18.4(i)0(n)18.4(g)0(.)]TJ
/F5 1 Tf
0 -2.447 TD
[(Materials)-332.7(and)-338.2(Methods)]TJ
/F10 1 Tf
0 -1.8336 TD
[(Coreshell)-336.6(ber)-332.9(processing)]TJ
/F3 1 Tf
1.1128 -1.8337 TD
[(A)-368.1(dual)-367.8(concentric)-372.4(nozzle)-369(\(inner)-365.5(23G)-370.8(and)-366.3(outer)-365.6(17G\))-371.3(was)]TJ
-1.1128 -1.2203 TD
[(specically)-682.6(designed)-682.4(and)-676.1(used)-682.1(to)-673.5(produce)-684.9(a)-676.1(coreshell)]TJ
T*
[(structured)-318.4(brous)-310.7(network)-310.4(of)-313.1(collagen-alginate.)-318.5(The)-309.8(sodium)]TJ
0 -1.2266 TD
[(alginate)-393.6(used)-391.3(\(A2158;)-388.5(Sigma-Aldrich\))-392.3(had)-391.6(an)-388.6(M/G)-386.2(ratio)-392.7(of)]TJ
0 -1.2203 TD
[(1.67)-475.7(and)-473.8(a)-473.7(molecular)-482.4(weight)-474.9(of)]TJ
/F4 1 Tf
15.0359 0 TD
(*)Tj
/F3 1 Tf
.999 0 TD
[(50,000)-474.7(Da.)-478.1(Solutions)-479.2(of)]TJ
-16.0349 -1.2203 TD
[(sodium)-275.1(alginate)-279.8(at)-278.2(ratios)-273.9(of)-275.1(2%5%)-280(\(%)-274(wt\))-274.5(in)-278.8(water)-274.1(were)-280.3(fed)]TJ
0 -1.2266 TD
[(into)-383.2(the)-383.1(outer)-378.2(syringe,)-381(while)-385(the)-376.8(collagen)-383.1(solution)-385.9(was)-375.5(loa-)]TJ
0 -1.2203 TD
[(ded)-505.4(in)-512.8(the)-509.6(inner)-510.4(syringe.)-507.5(The)-505.8(schematic)-516.4(representation)-510.5(is)]TJ
T*
[(shown)-448.8(in)-443.2(Figure)-444.3(1a.)-444.5(The)-448.9(collagen)-446.3(\(rat)-443.8(tail)-449.6(type)-442.4(I)-444.7(collagen;)]TJ
T*
[(First)-465.7(Link;)-459.9(2.05)-165.9(mg/mL\))-461.3(solution)-461.7(was)-464(prepared)-461.7(by)-464.1(mixing)]TJ
0 -1.2266 TD
[(1)-170.2(m)0(L)-455.8(o)0(f)-464.8(collagen)-465.3(with)-464.3(100)]TJ
/F4 1 Tf
12.1653 0 TD
(m)Tj
/F3 1 Tf
.5564 0 TD
.4583 Tc
[(Lo)458.3(f)-6.5(1)458.3(0)]TJ
/F9 1 Tf
3.5535 0 TD
0 Tc
()Tj
/F3 1 Tf
1.1824 0 TD
[(Dulbeccos)-464.1(modied)]TJ
-17.4576 -1.2203 TD
[(Eagle)-794.4(medium)-793.3(and)-796.3(a)-796.2(proper)-790.8(amount)-801.8(of)-793.6(1)-163.9(N)-796.7(sodium)]TJ
T*
[(hydroxide)-433.9(to)-426.9(provide)-429.3(a)-429.5(neutral)-432.5(pH)-425(to)-433.2(adjust)-427.9(solution)-430.1(con-)]TJ
0 -1.2267 TD
[(ditions)-517.5(for)-516.1(subsequent)-517.3(gelation)-510.7(and)-518.1(providing)-516.6(a)-511.7(nontoxic)]TJ
27.9978 74.5598 TD
[(environment)-319.2(for)-307.4(the)-313.6(loaded)-308.9(cells.)-314(Each)-308.7(syringe)-314.8(was)-306(attached)]TJ
0 -1.2203 TD
[(to)-483.8(an)-483.4(injection)-482.8(pump)-482.2(\(KD)-478.9(Scientic\))-485.6(connected)-485.6(through)-484.4(a)]TJ
0 -1.2266 TD
[(microtube)-585.4(and)-575(the)-579.1(injection)-584(rate)-582.3(varied)-576.4(between)-578.7(20)-580.7(and)]TJ
0 -1.2203 TD
[(80)-169.7(mL/h.)-281.8(Coreshell)-292.9(structured)-286.8(collagen-alginate)-290.3(bers)-287.5(were)]TJ
T*
[(then)-348.6(injected)-343.2(through)-345.3(the)-345.2(concentric)-347.1(nozzle)-343.7(into)-345.3(a)-340.9(bath)-349.2(con-)]TJ
0 -1.2266 TD
[(taining)-277.5(50)-163.4(mM)-274.6(calcium)-269.6(chloride)-275.3(\(CaCl)]TJ
6.7246 0 0 5.9768 462.3306 669.4865 Tm
(2)Tj
8.9663 0 0 8.9663 465.6755 670.9604 Tm
[(\))-272.4(for)-275.8(5)-163.9(min.)-273(The)-271.8(whole)]TJ
-17.0213 -1.2203 TD
[(injection)-546(process)-544.1(was)-539.9(performed)-543.8(under)-539.5(sterile)-540.5(conditions.)]TJ
T*
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