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Comparison of olfactory bulbar and mucosal cultures in a rat rhizotomy model

Ibrahim, A; Li, D; Collins, A; Tabakow, P; Raisman, G; Li, Y; (2014) Comparison of olfactory bulbar and mucosal cultures in a rat rhizotomy model. Cell Transplantation , 23 (11) pp. 1465-1470. 10.3727/096368913X676213. Green open access

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Abstract

In an ongoing clinical trial, a spinal injured patient who received a transplant of autologous cells cultured from the olfactory bulb is showing greater functional benefit than three previous patients with transplants of mucosal origin. Previous laboratory studies of transplantation into rat spinal cord injuries show that the superior reparative benefits of bulbar over mucosal cultures are associated with regeneration of severed corticospinal tract fibers over a bridge of olfactory ensheathing cells (OECs) formed across the injury site. In a rat rhizotomy paradigm, we reported that transplantation of bulbar cell cultures also enables severed axons of the C6-T1 dorsal roots to regenerate across a bridge of OECs into the spinal cord and restore electrophysiological transmission and forepaw grasping during a climbing test. We now report a repeat of the same rhizotomy procedure in 25 rats receiving cells cultured from olfactory mucosal biopsies. In no case did the transplanted cells form a bridging pathway. No axons crossed from the severed roots to the spinal cord, and there was no restoration of forepaw grasping. This suggests that the superior clinical benefit in the patient receiving bulbar cell transplants is due to regeneration of severed fibers across the injury site, and this correlates with imaging and the pattern of functional recovery. Using present culture protocols, the yield of OECs from bulbar biopsies is around 50%, but that from mucosal biopsies is less than 5%. Improving the yield of OECs from mucosal biopsies might avoid the necessity for the intracranial approach to obtain bulbar cells.

Type: Article
Title: Comparison of olfactory bulbar and mucosal cultures in a rat rhizotomy model
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.3727/096368913X676213
Publisher version: http://dx.doi.org/10.3727/096368913X676213
Language: English
Additional information: This is an Open Access article published under a Creative Commons Attribution Non-Commercial (CC BY NC) license (http://creativecommons.org/licenses/by-nc/3.0/).
Keywords: Animals, Cell Transplantation, Cells, Cultured, Disease Models, Animal, Female, Olfactory Bulb, Olfactory Mucosa, Rats, Rhizotomy, Spinal Cord Injuries
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Brain Repair and Rehabilitation
URI: https://discovery.ucl.ac.uk/id/eprint/1423663
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