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Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours.

Kuipers, D; Mehonic, A; Kajita, M; Peter, L; Fujita, Y; Duke, T; Charras, G; (2014) Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours. J Cell Sci 10.1242/jcs.138289. Green open access

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Abstract

Epithelial cells maintain an essential barrier despite continuously undergoing mitosis and apoptosis. Biological and biophysical mechanisms have evolved to remove dying cells whilst maintaining that barrier. Cell extrusion is thought to be driven by a multicellular filamentous actin ring formed by the neighbouring cells, with its contraction providing the mechanical force for extrusion, with little or no contribution from the dying cell. We use live confocal imaging, providing time-resolved 3D observations of actomyosin dynamics to reveal new mechanical roles for dying cells in their own extrusion from monolayers. Dying cell clearance could be subdivided into two-stages. The first, previously unidentified, stage was driven by the dying cell, which exerted tension on its neighbours through the action of a cortical contractile F-actin and myosin ring at the cell apex. The second stage, consistent with previous studies, was driven by a multicellular F-actin ring in the neighbouring cells that moved from the apical to the basal plane to extrude the dying cell. Critically, these data reinstate the dying cell as an active physical participant in cell extrusion rather than an innocent bystander.

Type: Article
Title: Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.138289
Publisher version: http://dx.doi.org/10.1242/jcs.138289
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > The Ear Institute
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Med Phys and Biomedical Eng
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > London Centre for Nanotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/1419244
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