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Peptidome analysis of cerebrospinal fluid by LC-MALDI MS

Hölttä, M; Zetterberg, H; Mirgorodskaya, E; Mattsson, N; Blennow, K; Gobom, J; (2012) Peptidome analysis of cerebrospinal fluid by LC-MALDI MS. PLoS One , 7 (8) , Article e42555. 10.1371/journal.pone.0042555. Green open access

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Abstract

We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000-4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.

Type: Article
Title: Peptidome analysis of cerebrospinal fluid by LC-MALDI MS
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0042555
Publisher version: http://dx.doi.org/10.1371/journal.pone.0042555
Language: English
Additional information: © Hölttä et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3412831
Keywords: Amino Acid Sequence, Cerebrospinal Fluid Proteins, Chromatography, Liquid, Databases, Protein, Humans, Molecular Sequence Data, Peptides, Proteome, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/1411965
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