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Aβ40 oligomers identified as a potential biomarker for the diagnosis of Alzheimer's disease

Gao, CM; Yam, AY; Wang, X; Magdangal, E; Salisbury, C; Peretz, D; Zuckermann, RN; ... Allauzen, S; + view all (2010) Aβ40 oligomers identified as a potential biomarker for the diagnosis of Alzheimer's disease. PLoS One , 5 (12) , Article e15725. 10.1371/journal.pone.0015725. Green open access

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Abstract

Alzheimer's Disease (AD) is the most prevalent form of dementia worldwide, yet the development of therapeutics has been hampered by the absence of suitable biomarkers to diagnose the disease in its early stages prior to the formation of amyloid plaques and the occurrence of irreversible neuronal damage. Since oligomeric Aβ species have been implicated in the pathophysiology of AD, we reasoned that they may correlate with the onset of disease. As such, we have developed a novel misfolded protein assay for the detection of soluble oligomers composed of Aβ x-40 and x-42 peptide (hereafter Aβ40 and Aβ42) from cerebrospinal fluid (CSF). Preliminary validation of this assay with 36 clinical samples demonstrated the presence of aggregated Aβ40 in the CSF of AD patients. Together with measurements of total Aβ42, diagnostic sensitivity and specificity greater than 95% and 90%, respectively, were achieved. Although larger sample populations will be needed to confirm this diagnostic sensitivity, our studies demonstrate a sensitive method of detecting circulating Aβ40 oligomers from AD CSF and suggest that these oligomers could be a powerful new biomarker for the early detection of AD.

Type: Article
Title: Aβ40 oligomers identified as a potential biomarker for the diagnosis of Alzheimer's disease
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0015725
Publisher version: http://dx.doi.org/10.1371/journal.pone.0015725
Language: English
Additional information: © 2010 Gao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3012719
Keywords: Aged, Alzheimer Disease, Amyloid beta-Peptides, Biological Markers, Brain, Enzyme-Linked Immunosorbent Assay, Epitopes, Gene Expression Regulation, Humans, Middle Aged, Models, Chemical, Molecular Conformation, Peptide Fragments, Peptides, Protein Conformation, Sensitivity and Specificity
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/1411864
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