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Microfluidic multi-input reactor for biocatalytic synthesis using transketolase

Lawrence, J; O'Sullivan, B; Lye, GJ; Wohlgemuth, R; Szita, N; (2013) Microfluidic multi-input reactor for biocatalytic synthesis using transketolase. Journal of Molecular Catalysis B: Enzymatic , 95 111 - 117. 10.1016/j.molcatb.2013.05.016. Green open access

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Biocatalytic synthesis in continuous-flow microreactors is of increasing interest for the production of specialty chemicals. However, the yield of production achievable in these reactors can be limited by the adverse effects of high substrate concentration on the biocatalyst, including inhibition and denaturation. Fed-batch reactors have been developed in order to overcome this problem, but no continuous-flow solution exists. We present the design of a novel multi-input microfluidic reactor, capable of substrate feeding at multiple points, as a first step towards overcoming these problems in a continuous-flow setting. Using the transketolase-(TK) catalysed reaction of lithium hydroxypyruvate (HPA) and glycolaldehyde (GA) to l-erythrulose (ERY), we demonstrate the transposition of a fed-batch substrate feeding strategy to our microfluidic reactor. We obtained a 4.5-fold increase in output concentration and a 5-fold increase in throughput compared with a single input reactor.

Type: Article
Title: Microfluidic multi-input reactor for biocatalytic synthesis using transketolase
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.molcatb.2013.05.016
Publisher version: http://dx.doi.org/10.1016/j.molcatb.2013.05.016
Language: English
Additional information: © 2013 Elsevier B.V. except certain content provided by third parties. ScienceDirect® is a registered trademark of Elsevier B.V. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1403743
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