Nathani, RI;
Moody, P;
Chudasama, V;
Smith, MEB;
Fitzmaurice, RJ;
Caddick, S;
(2013)
A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification.
Chemical Science
, 4
(9)
pp. 3455-3458.
10.1039/C3SC51333E.
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Abstract
Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.
| Type: | Article |
|---|---|
| Title: | A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/C3SC51333E |
| Publisher version: | http://dx.doi.org/10.1039/C3SC51333E |
| Language: | English |
| Additional information: | © The Royal Society of Chemistry 2013. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1400123 |
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