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Differential Regulation of SC1/PRDM4 and PRMT5 mediated protein arginine methylation by the Nerve Growth Factor and the Epidermal Growth Factor in PC12 cells

Chittka, A; (2013) Differential Regulation of SC1/PRDM4 and PRMT5 mediated protein arginine methylation by the Nerve Growth Factor and the Epidermal Growth Factor in PC12 cells. Neuroscience Letters , 550 87- 92. 10.1016/j.neulet.2013.06.051. Green open access

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Abstract

During neuronal development, the neuroepithelial stem cells (NSCs) initially undergo proliferative divisions, later switching to neurogenic ones whereby one NSC and a post-mitotic neuron are generated. We recently showed that a member of the PRDM family of transcriptional regulators, PRDM4/SC1, recruits a type II protein arginine methyltransferase, PRMT5, to maintain the "stem-like" cellular state of the embryonic mouse cortical NSCs. However, little is known about the regulation of activity of this complex under proliferation- or differentiation-inducing growth conditions. In the present work I investigate the regulation of SC1/PRMT5-mediated methylation activity in PC12 cells treated with EGF or NGF. I present evidence that NGF down-regulates SC1/PRMT5 methyltransferase (MTase) activity and that the reduction in SC1/PRMT5 MTase activity occurs mainly in the nucleus. I suggest that high levels of SC1/PRMT5 activity are associated with the proliferative state of the cells.

Type: Article
Title: Differential Regulation of SC1/PRDM4 and PRMT5 mediated protein arginine methylation by the Nerve Growth Factor and the Epidermal Growth Factor in PC12 cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.neulet.2013.06.051
Publisher version: http://dx.doi.org/10.1016/j.neulet.2013.06.051
Language: English
Additional information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: (H)MTase, (histone) methyltransferase, EGF, Histone arginine methylation, NGF, NSC, PC12 cells, PRDM, PRDM4, PRMT, PRMT5, SC1, Schwann cell factor 1, epidermal growth factor, nerve growth factor, neural stem cells, positive regulatory domain protein, protein arginine methyltransferase
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/1399195
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