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Bacterial Infection Elicits Heat Shock Protein 72 Release from Pleural Mesothelial Cells

della Vergiliana, JFV; Lansley, SM; Porcel, JM; Bielsa, S; Brown, JS; Creaney, J; Temple, SEL; ... Lee, YCG; + view all (2013) Bacterial Infection Elicits Heat Shock Protein 72 Release from Pleural Mesothelial Cells. PLOS ONE , 8 (5) , Article e63873. 10.1371/journal.pone.0063873. Green open access

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Abstract

Heat shock protein 70 (HSP70) has been implicated in infection-related processes and has been found in body fluids during infection. This study aimed to determine whether pleural mesothelial cells release HSP70 in response to bacterial infection in vitro and in mouse models of serosal infection. In addition, the in vitro cytokine effects of the HSP70 isoform, Hsp72, on mesothelial cells were examined. Further, Hsp72 was measured in human pleural effusions and levels compared between non-infectious and infectious patients to determine the diagnostic accuracy of pleural fluid Hsp72 compared to traditional pleural fluid parameters. We showed that mesothelial release of Hsp72 was significantly raised when cells were treated with live and heat-killed Streptococcus pneumoniae. In mice, intraperitoneal injection of S. pneumoniae stimulated a 2-fold increase in Hsp72 levels in peritoneal lavage (p<0.01). Extracellular Hsp72 did not induce or inhibit mediator release from cultured mesothelial cells. Hsp72 levels were significantly higher in effusions of infectious origin compared to non-infectious effusions (p<0.05). The data establish that pleural mesothelial cells can release Hsp72 in response to bacterial infection and levels are raised in infectious pleural effusions. The biological role of HSP70 in pleural infection warrants exploration.

Type: Article
Title: Bacterial Infection Elicits Heat Shock Protein 72 Release from Pleural Mesothelial Cells
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0063873
Publisher version: http://dx.doi.org/10.1371/journal.pone.0063873
Language: English
Additional information: © 2013 Varano della Vergiliana et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/1398755
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