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Evaluation of Methods for Sizing and Counting of Ultrasound Contrast Agents

Sennoga, CA; Yeh, JSM; Alter, J; Stride, E; Nihoyannopoulos, P; Seddon, JM; Haskard, DO; ... Eckersley, RJ; + view all (2012) Evaluation of Methods for Sizing and Counting of Ultrasound Contrast Agents. Ultrasound in Medicine & Biology , 38 (5) 834 - 845. 10.1016/j.ultrasmedbio.2012.01.012. Green open access

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Abstract

A precise, accurate and well documented method for the sizing and counting of microbubbles is essential for all aspects of quantitative microbubble-enhanced ultrasound imaging. The efficacy of (a) electro-impedance volumetric zone sensing (ES) also called a Coulter counter/multisizer; (b) optical microscopy (OM); and (c) laser diffraction (LD), for the sizing and counting of microbubbles was assessed. Microspheres with certified mean diameter and number concentration were used to assess sizing and counting reproducibility (precision) and reliability (accuracy) of ES, OM and LD. SonoVueTM was repeatedly (n 5 3) sized and counted to validate ES, OM and LD sizing and counting efficacy. Statistical analyses of intra-method variability for the SonoVueTM mean diameter showed that the best microbubble sizing reproducibility was obtained usingOMwith a mean diameter sizing variability of 1.1%, compared with a variability of 4.3% for ES and 7.1% for LD. The best microbubble counting reproducibility was obtained using ES with a number concentration variability of 8.3%, compared with a variability of 22.4% forOMand 32% for LD. This study showed that no method is fully suited to both sizing and counting of microbubbles.

Type: Article
Title: Evaluation of Methods for Sizing and Counting of Ultrasound Contrast Agents
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ultrasmedbio.2012.01.012
Publisher version: http://dx.doi.org/10.1016/j.ultrasmedbio.2012.01.0...
Language: English
Additional information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Security and Crime Science
URI: https://discovery.ucl.ac.uk/id/eprint/1398676
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