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Assessing predictors of changes in protein stability upon mutation using self-consistency

Thiltgen, G; Goldstein, RA; (2012) Assessing predictors of changes in protein stability upon mutation using self-consistency. PLoS One , 7 (10) , Article e46084. 10.1371/journal.pone.0046084. Green open access

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Abstract

The ability to predict the effect of mutations on protein stability is important for a wide range of tasks, from protein engineering to assessing the impact of SNPs to understanding basic protein biophysics. A number of methods have been developed that make these predictions, but assessing the accuracy of these tools is difficult given the limitations and inconsistencies of the experimental data. We evaluate four different methods based on the ability of these methods to generate consistent results for forward and back mutations, and examine how this ability varies with the nature and location of the mutation. We find that, while one method seems to outperform the others, the ability of these methods to make accurate predictions is limited.

Type: Article
Title: Assessing predictors of changes in protein stability upon mutation using self-consistency
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0046084
Publisher version: http://dx.doi.org/10.1371/journal.pone.0046084
Language: English
Additional information: © 2012 Thiltgen, Goldstein. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3483175
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
URI: https://discovery.ucl.ac.uk/id/eprint/1396082
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