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Kv7/M-type potassium channels in rat skin keratinocytes.

Reilly, JM; Telezhkin, V; Passmore, GM; Marsh, SJ; Brown, DA; (2013) Kv7/M-type potassium channels in rat skin keratinocytes. Pflügers Archiv - European Journal of Physiology 10.1007/s00424-013-1276-2. Green open access

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Abstract

Skin keratinocytes fulfil important signalling and protective functions. Immunocytochemical experiments revealed the unexpected presence of immunoreactivity for the M-type potassium channel subunit Kv7.2 in the keratinocyte layer of intact rat paw skin and in keratinocytes isolated from the skin of 1-day-old rats and cultured in vitro for 3-10 days. Application of the M-channel enhancer retigabine (3-10 μM) to isolated cultured rat keratinocytes: (a) increased outward membrane currents recorded under voltage clamp, (b) produced ~3 mV hyperpolarization at rest, (c) enhanced ~3-fold the release of ATP induced by the TRPV3 agonist carvacrol (1 mM) and (d) increased the amplitude of the carvacrol-induced intracellular Ca(2+) transient measured with Fura-2. The effect of retigabine on ATP release was prevented by the M-channel blocking agent XE991. We conclude that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, with potential significance for their sensory and other biological functions.

Type: Article
Title: Kv7/M-type potassium channels in rat skin keratinocytes.
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/s00424-013-1276-2
Publisher version: http://dx.doi.org/10.1007/s00424-013-1276-2
Language: English
Additional information: © The Author(s) 2013. This article is published with open access at Springerlink.com
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Neuro, Physiology and Pharmacology
URI: https://discovery.ucl.ac.uk/id/eprint/1393640
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