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A novel tissue engineered three-dimensional in vitro colorectal cancer model

Nyga, A; Loizidou, M; Emberton, M; Cheema, U; (2013) A novel tissue engineered three-dimensional in vitro colorectal cancer model. Acta Biomaterialia , 9 (8) pp. 7917-7926. 10.1016/j.actbio.2013.04.028. Green open access

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Abstract

The interactions of cancer cells within a solid mass with the surrounding reactive stroma are critical for growth and progression. The surrounding vasculature is recruited into the periphery of the growing tumour to supply cancer cells with nutrients and O2. This study focuses on developing a novel three-dimensional (3-D) in vitro biomimetic colorectal cancer model using colorectal cancer cells and connective tissue cells. The 3-D model comprises a dense artificial cancer mass, created by partial plastic compression of collagen type I containing HT29 colorectal cancer cells, nested in a non-dense collagen type I gel populated by fibroblasts and/or endothelial cells. HT29 cells within the dense mass proliferate slower than when cultured in a two-dimensional system. These cells form tumour spheroids which invade the surrounding matrix, away from the hypoxic conditions in the core of the construct, measured using real time O2 probes. This model is also characterized by the release of vascular endothelial growth factor (VEGF) by HT29 cells, mainly at the invading edge of the artificial cancer mass. This characterization is fundamental in establishing a reproducible, complex model that could be used to advance our understanding of cancer pathology and will facilitate therapeutic drug testing.

Type: Article
Title: A novel tissue engineered three-dimensional in vitro colorectal cancer model
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.actbio.2013.04.028
Publisher version: http://dx.doi.org/10.1016/j.actbio.2013.04.028
Language: English
Additional information: © 2013 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V. This work is licensed under the Creative Commons Attribution License, Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/ which permits unrestricted use, distribution, and reproduction in any medium. However, you must attribute the work to the author (but not in any way that suggests that they endorse you or your use of the work).
Keywords: Collagen I, Hypoxia, Plastic compression, Three-dimensional culture, Tumour microenvironment, Tumour invasion
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/1392632
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