Chen, Y;
Ogundare, M;
Williams, CM;
Wang, Y;
Wang, Y;
Sewell, GW;
Smith, PJ;
... Griffiths, WJ; + view all
(2013)
Shotgun cholanomics of ileal fluid.
Biochimie
, 95
(3)
461 - 463.
10.1016/j.biochi.2012.09.004.
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Abstract
In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn's disease. In a pilot study we demonstrate the method by analysing the bile acid content of ileal fluid from seven Crohn's disease patients and three healthy controls. The dominant bile acids observed were di and/or trihydroxycholanoates, di- and/or trihydroxycholanoylglycines, di- and/or tri-hydroxycholanoyltaurines, monosulphated dihydroxycholanoates and monosulphated dihydroxycholanoylglycine. The method can be similarly applied to samples derived from other parts of the intestine.
| Type: | Article |
|---|---|
| Title: | Shotgun cholanomics of ileal fluid |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.biochi.2012.09.004 |
| Publisher version: | http://dx.doi.org/10.1016/j.biochi.2012.09.004 |
| Language: | English |
| Additional information: | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PubMed ID: 22986022 |
| Keywords: | Bile acid, Lipidomics, Mass spectrometry, Electrospray ionization, Crohn's disease |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1391124 |
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