Cowper, B;
Craik, DJ;
Macmillan, D;
(2013)
Making ends meet: chemically mediated circularization of recombinant proteins.
Chembiochem
, 14
(7)
809 - 812.
10.1002/cbic.201300105.
![[thumbnail of 809_ftp.pdf]](https://discovery.ucl.ac.uk/style/images/fileicons/application_pdf.png) Preview |
PDF
809_ftp.pdf Download (794kB) |
Abstract
A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.
| Type: | Article |
|---|---|
| Title: | Making ends meet: chemically mediated circularization of recombinant proteins. |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/cbic.201300105 |
| Publisher version: | http://dx.doi.org/10.1002/cbic.201300105 |
| Language: | English |
| Additional information: | � 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. PMCID: PMC4016753 |
| Keywords: | Cyclization, Cyclotides, Recombinant Proteins |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1389745 |
Archive Staff Only
 |
View Item |
