UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Oxygen-controlled automated neural differentiation of mouse embryonic stem cells.

Mondragon-Teran, P; Tostoes, R; Mason, C; Lye, GJ; Veraitch, FS; (2013) Oxygen-controlled automated neural differentiation of mouse embryonic stem cells. Regen Med , 8 (2) 171 - 182. 10.2217/rme.13.12. Green open access

[img]
Preview
PDF
Farlan_Automated_mESC_neural_differentiation.pdf

Download (505kB)

Abstract

Automation and oxygen tension control are two tools that provide significant improvements to the reproducibility and efficiency of stem cell production processes. Aim: the aim of this study was to establish a novel automation platform capable of controlling oxygen tension during both the cell-culture and liquid-handling steps of neural differentiation processes. Materials & methods: We built a bespoke automation platform, which enclosed a liquid-handling platform in a sterile, oxygen-controlled environment. An airtight connection was used to transfer cell culture plates to and from an automated oxygen-controlled incubator. Results: Our results demonstrate that our system yielded comparable cell numbers, viabilities, metabolism profiles and differentiation efficiencies when compared with traditional manual processes. Interestingly, eliminating exposure to ambient conditions during the liquid-handling stage resulted in significant improvements in the yield of MAP2-positive neural cells, indicating that this level of control can improve differentiation processes. Conclusion: This article describes, for the first time, an automation platform capable of maintaining oxygen tension control during both the cell-culture and liquid-handling stages of a 2D embryonic stem cell differentiation process.

Type: Article
Title: Oxygen-controlled automated neural differentiation of mouse embryonic stem cells.
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.2217/rme.13.12
Publisher version: http://dx.doi.org/10.2217/rme.13.12
Language: English
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1389447
Downloads since deposit
189Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item