Nathani, RI;
Chudasama, V;
Ryan, CP;
Moody, PR;
Morgan, RE;
Fitzmaurice, RJ;
Smith, ME;
... Caddick, S; + view all
(2013)
Reversible protein affinity-labelling using bromomaleimide-based reagents.
Organic & Biomolecular Chemistry
, 11
(15)
2408 -2411.
10.1039/c3ob40239h.
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Abstract
Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.
| Type: | Article |
|---|---|
| Title: | Reversible protein affinity-labelling using bromomaleimide-based reagents |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/c3ob40239h |
| Publisher version: | http://dx.doi.org/10.1039/c3ob40239h |
| Language: | English |
| Additional information: | This work is licensed under the Creative Commons Attribution License, Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/ which permits unrestricted use, distribution, and reproduction in any medium. However, you must attribute the work to the author (but not in any way that suggests that they endorse you or your use of the work). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1388302 |
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