Fletcher, AL;
Lukacs-Kornek, V;
Reynoso, ED;
Pinner, SE;
Bellemare-Pelletier, A;
Curry, MS;
Collier, A-R;
... Turley, SJ; + view all
(2010)
Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions.
Journal of Experimental Medicine
, 207
(4)
pp. 689-697.
10.1084/jem.20092642.
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Abstract
Lymph node stromal cells (LNSCs) can induce potent, antigen-specific T cell tolerance under steady-state conditions. Although expression of various peripheral tissue–restricted antigens (PTAs) and presentation to naive CD8+ T cells has been demonstrated, the stromal subsets responsible have not been identified. We report that fibroblastic reticular cells (FRCs), which reside in the T cell zone of the LN, ectopically express and directly present a model PTA to naive T cells, inducing their proliferation. However, we found that no single LNSC subset was responsible for PTA expression; rather, each subset had its own characteristic antigen display. Studies to date have concentrated on PTA presentation under steady-state conditions; however, because LNs are frequently inflammatory sites, we assessed whether inflammation altered stromal cell–T cell interactions. Strikingly, FRCs showed reduced stimulation of T cells after Toll-like receptor 3 ligation. We also characterize an LNSC subset expressing the highest levels of autoimmune regulator, which responds potently to bystander inflammation by up-regulating PTA expression. Collectively, these data show that diverse stromal cell types have evolved to constitutively express PTAs, and that exposure to viral products alters the interaction between T cells and LNSCs.
| Type: | Article |
|---|---|
| Title: | Lymph node fibroblastic reticular cells directly present peripheral tissue antigen under steady-state and inflammatory conditions |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1084/jem.20092642 |
| Publisher version: | https://doi.org/10.1084/jem.20092642 |
| Language: | English |
| Additional information: | Copyright © 2010 Fletcher et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/ licenses/by-nc-sa/3.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1387307 |
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