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Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH.

Werbeck, ND; Kirkpatrick, J; Hansen, DF; (2013) Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH. Angew Chem Int Ed Engl , 52 (11) 3145 - 3147. 10.1002/anie.201209385. Green open access

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Abstract

Arginine side-chains play a distinct role because of their high pKa and perpetual positive charge. An NMR method is presented, based on carbon-detected 13Cζ–15Nε correlation spectra, which allows probing the arginine side-chains and their dynamics at neutral-to-high pH. The methodology is demonstrated on human histone deacetylase 8.

Type: Article
Title: Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH.
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/anie.201209385
Publisher version: http://dx.doi.org/10.1002/anie.201209385
Language: English
Additional information: �© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. PMCID: PMC4016738
Keywords: Arginine, Carbon, Histone Deacetylases, Humans, Hydrogen-Ion Concentration, Models, Molecular, Nuclear Magnetic Resonance, Biomolecular, Repressor Proteins
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/1386411
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