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Production of Soluble Human Vascular Endothelial Growth Factor VEGF-A(165)-Heparin Binding Domain in Escherichia coli.

Seyedarabi, A; Cheng, L; Zachary, I; Djordjevic, S; (2013) Production of Soluble Human Vascular Endothelial Growth Factor VEGF-A(165)-Heparin Binding Domain in Escherichia coli. PLoS One , 8 (2) , Article e55690. 10.1371/journal.pone.0055690. Green open access

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Abstract

We report a method for production of soluble heparin binding domain (HBD) of human vascular endothelial growth factor VEGF-A(165). Recombinant VEGF-A(165)-HBD that contains four disulphide bridges was expressed in specialised E. coli SHuffle cells and its activity has been confirmed through interactions with neuropilin and heparin. The ability to produce significant quantities of a soluble active form of VEGF-A(165)-HBD will enable further studies addressing the role of VEGF-A in essential processes such as angiogenesis, vasculogenesis and vascular permeability.

Type: Article
Title: Production of Soluble Human Vascular Endothelial Growth Factor VEGF-A(165)-Heparin Binding Domain in Escherichia coli.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0055690
Publisher version: http://dx.doi.org/10.1371/journal.pone.0055690
Language: English
Additional information: © 2013 Seyedarabi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3568127
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Metabolism and Experi Therapeutics
URI: https://discovery.ucl.ac.uk/id/eprint/1386314
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