Ruchhoeft, ML;
Ohnuma, S;
McNeill, L;
Holt, CE;
Harris, WA;
(1999)
The neuronal architecture of Xenopus retinal ganglion cells is sculpted by rho-family GTPases in vivo.
JOURNAL OF NEUROSCIENCE
, 19
(19)
8454 - 8463.
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Abstract
Dendritogenesis, axonogenesis, pathfinding, and target recognition are all affected in distinct ways when Xenopus retinal ganglion cells (RGCs) are transfected with constitutively active (ca), wild-type (wt), and dominant negative (dn) Rho-family GTPases in vivo. Dendritogenesis required Rac1 and Cdc42 activity. Moreover, ca-Rac1 caused dendrite hyperproliferation. Axonogenesis, in contrast, was inhibited by ca-Rac1. This phenotype was partially rescued by the coexpression of dn cyclin-dependent kinase (Cdk5), a proposed effector of Rac1, suggesting that Rac1 activity must be regulated tightly for normal axonogenesis. Growth cone morphology was particularly sensitive to dn-RhoA and wt-Cdc42 constructs. These also caused targeting errors, such as tectal bypass, suggesting that cytoskeletal rearrangements are involved in target recognition and are transduced by these pathways.
| Type: | Article |
|---|---|
| Title: | The neuronal architecture of Xenopus retinal ganglion cells is sculpted by rho-family GTPases in vivo |
| Open access status: | An open access version is available from UCL Discovery |
| Publisher version: | http://www.jneurosci.org/content/19/19/8454 |
| Language: | English |
| Additional information: | This work is licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The license allows you to copy, distribute, and transmit the work, as well as adapting it. However, you must attribute the work to the author (but not in any way that suggests that they endorse you or your use of the work), and cannot use the work for commercial purposes without prior permission of the author. If you alter or build upon this work, you can distribute the resulting work only under the same or similar license to this one. PubMed ID: 10493746 |
| Keywords: | RhoA, Rac1, Cdc42, axonogenesis, dendritogenesis, GTPase |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1372851 |
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