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Control of directed cell migration in vivo by membrane-to-cortex attachment.

Diz-Muñoz, A; Krieg, M; Bergert, M; Ibarlucea-Benitez, I; Muller, DJ; Paluch, E; Heisenberg, CP; (2010) Control of directed cell migration in vivo by membrane-to-cortex attachment. PLoS Biol , 8 (11) , Article e1000544. 10.1371/journal.pbio.1000544. Green open access

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Abstract

Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.

Type: Article
Title: Control of directed cell migration in vivo by membrane-to-cortex attachment.
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pbio.1000544
Publisher version: http://dx.doi.org/10.1371/journal.pbio.1000544
Language: English
Additional information: © 2010 Diz-Muñoz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This work was supported by grants from the BIF to MK, the Polish Ministry of Science and Higher Education to EP, and the DFG (HE 3231/6-1and PA 1590/1-1) to CPH and EP. The Deutsche Forschungsgemeinschaft, Max-Planck-Gesellschaft, Institute of Science and Technology Austria also supported this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Keywords: Animals, Cell Membrane, Cell Movement, Cytoskeleton, Gastrulation, Mesoderm, Microscopy, Atomic Force, Microscopy, Confocal, Pseudopodia, Stem Cells, Zebrafish
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL
URI: https://discovery.ucl.ac.uk/id/eprint/1371992
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