Williams, AE;
Perry, MM;
Moschos, SA;
Lindsay, MA;
(2007)
microRNA expression in the aging mouse lung.
BMC Genomics
, 8
, Article 172. 10.1186/1471-2164-8-172.
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Abstract
BACKGROUND: MicroRNAs (miRNAs) are a novel class of short double stranded RNA that mediate the post-transcriptional regulation of gene expression. Previous studies have implicated changes in miRNA expression in the regulation of development and the induction of diseases such as cancer. However, although miRNAs have been implicated in the process of aging in C. elegans, nothing is known of their role in mammalian tissues. RESULTS: To address this question, we have used a highly-sensitive, semi-quantitative RT-PCR based approach to measure the expression profile of 256 of the 493 currently identified miRNAs in the lungs from 6 month (adult) and 18 month (aged) old female BALB/c mice. We show that, despite the characteristic changes in anatomy and gene expression associated with lung aging, there were no significant changes in the expression of 256 miRNAs. CONCLUSION: Overall, these results show that miRNA transcription is unchanged during lung aging and suggests that stable expression of miRNAs might instead buffer age related changes in the expression of protein-encoding genes
| Type: | Article |
|---|---|
| Title: | microRNA expression in the aging mouse lung |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/1471-2164-8-172 |
| Publisher version: | http://dx.doi.org/10.1186/1471-2164-8-172 |
| Language: | English |
| Additional information: | © 2007 Williams et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Adult, Aging, Animals, Female, Gene Expression Profiling, genetics, Lung, metabolism, Mice, Mice,Inbred BALB C, MicroRNAs, Reverse Transcriptase Polymerase Chain Reaction |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Department of Education |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1360189 |
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