Cheema, U;
Alp, B;
Brown, RA;
MacRobert, AJ;
(2006)
Perfusion of oxygen in 3D plastic compressed collagen constructs.
In:
Abstracts of the Tissue and Cell Engineering Society (TCES) meeting 3-4 July 2006, University of Sheffield.
(pp. 21 - 21).
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Abstract
The development of 3D connective tissues in vitro is heavily dependent upon remodelling of the matrix, in particular collagen, by resident cells. We have developed a novel plastic compression (PC) technique, for the fabrication of dense cell-collagen based bio-mimetic tissues (Brown et al. 2005). Cell survival in these PC collagen constructs is critical for successful tissue modelling and so the aim here is to understand, quantitatively their dynamic perfusion. This is important for the development of tissue bioreactors for the culture of PC constructs. We have used a fibre-optic oxygen sensor to measure changing oxygen levels in the core of such constructs. This effectively measures O2 consumption by cells, and by extrapolation, gradients and diffusion properties in the model tissues, which can be correlated with cell death.
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