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Perfusion of oxygen in 3D plastic compressed collagen constructs

Cheema, U; Alp, B; Brown, RA; MacRobert, AJ; (2006) Perfusion of oxygen in 3D plastic compressed collagen constructs. In: Abstracts of the Tissue and Cell Engineering Society (TCES) meeting 3-4 July 2006, University of Sheffield. (pp. 21 - 21). Green open access

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Abstract

The development of 3D connective tissues in vitro is heavily dependent upon remodelling of the matrix, in particular collagen, by resident cells. We have developed a novel plastic compression (PC) technique, for the fabrication of dense cell-collagen based bio-mimetic tissues (Brown et al. 2005). Cell survival in these PC collagen constructs is critical for successful tissue modelling and so the aim here is to understand, quantitatively their dynamic perfusion. This is important for the development of tissue bioreactors for the culture of PC constructs. We have used a fibre-optic oxygen sensor to measure changing oxygen levels in the core of such constructs. This effectively measures O2 consumption by cells, and by extrapolation, gradients and diffusion properties in the model tissues, which can be correlated with cell death.

Type: Proceedings paper
Title: Perfusion of oxygen in 3D plastic compressed collagen constructs
Event: Tissue and Cell Engineering Society (TCES) meeting
Location: University of Sheffield
Dates: 03 July 2006-04 July 2006
Open access status: An open access version is available from UCL Discovery
Publisher version: http://www.ecmjournal.org/journal/supplements/vol0...
Language: English
Additional information: Reproduced with kind permission from eCM journal (www.ecmjournal.org)
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Ortho and MSK Science
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology
URI: https://discovery.ucl.ac.uk/id/eprint/1360161
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