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A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions

Caceres, C; Wright, G; Gouyette, C; Parkinson, G; Subirana, JA; (2004) A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions. NUCLEIC ACIDS RESEARCH , 32 (3) 1097 - 1102. 10.1093/nar/gkh269. Green open access

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Abstract

We report two new structures of the quadruplex d(TGGGGT)4 obtained by single crystal X‐ray diffraction. In one of them a thymine tetrad is found. Thus the yeast telomere sequences d(TG1–3) might be able to form continuous quadruplex structures, involving both guanine and thymine tetrads. Our study also shows substantial differences in the arrangement of thymines when compared with previous studies. We find five different types of organization: (i) groove binding with hydrogen bonds to guanines from a neighbour quadruplex; (ii) partially ordered groove binding, without any hydrogen bond; (iii) stacked thymine triads, formed at the 3′ends of the quadruplexes; (iv) a thymine tetrad between two guanine tetrads. Thymines are stabilized in pairs by single hydrogen bonds. A central sodium ion interacts with two thymines and contributes to the tetrad structure. (v) Completely disordered thymines which do not show any clear location in the crystal. The tetrads are stabilized by either Na+ or Tl+ ions. We show that by using MAD methods, Tl+ can be unambiguously located and distinguished from Na+. We can thus determine the preference for either ion in each ionic site of the structure under the conditions used by us.

Type: Article
Title: A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions
Open access status: An open access version is available from UCL Discovery
DOI: 10.1093/nar/gkh269
Publisher version: http://dx.doi.org/10.1093/nar/gkh269
Language: English
Additional information: PubMed ID: 14960719. © The Author(s) 2004. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1354286
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