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Transcutaneous immunisation assisted by low-frequency ultrasound.

Dahlan, A; Alpar, HO; Stickings, P; Sesardic, D; Murdan, S; (2009) Transcutaneous immunisation assisted by low-frequency ultrasound. Int J Pharm , 368 (1-2) 123 - 128. 10.1016/j.ijpharm.2008.10.014. Green open access

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Abstract

Low-frequency ultrasound application is known to increase the skin's permeability to large molecules such as vaccines, and to enable transcutaneous immunisation. Sodium dodecyl sulphate (SDS) - a skin irritant - is often included in the coupling medium at 1% (w/v), as this has been found to enhance skin permeability. In this paper we show, for the first time, the feasibility of low-frequency ultrasound-assisted transcutaneous immunisation in the absence of SDS. Antibody titres were strongly influenced by experimental conditions. SDS presence in the coupling medium increased antibody titres, though a lower concentration of 0.5% (w/v) generated much higher titres than the commonly used 1% (w/v), despite causing less skin damage. A lower ultrasound duty cycle of 10% generated higher antibody titres than a duty cycle of 20%, also despite causing lower skin damage. Such lack of correlation between skin damage and immune responses indicates that enhancement of skin permeability to topically applied antigen (as indicated by changes in skin integrity) was not the main mechanism of low-frequency ultrasound-assisted skin immunisation.

Type: Article
Title: Transcutaneous immunisation assisted by low-frequency ultrasound.
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.ijpharm.2008.10.014
Publisher version: http://dx.doi.org/10.1016/j.ijpharm.2008.10.014
Language: English
Additional information: NOTICE: this is the author’s version of a work that was accepted for publication in International Journal of Pharmaceutics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International Journal of Pharmaceutics Volume 368, Issues 1–2, 23 February 2009, Pages 123–128 http://dx.doi.org/10.1016/j.ijpharm.2008.10.014
Keywords: Administration, Cutaneous, Animals, Drug Delivery Systems, Female, Immunization, Mice, Mice, Inbred BALB C, Phonophoresis, Skin, Skin Absorption, Sodium Dodecyl Sulfate, Surface-Active Agents, Tetanus Toxoid
UCL classification: UCL
UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics
URI: https://discovery.ucl.ac.uk/id/eprint/1351481
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