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Analysis of myotube-motoneuron interaction within an in vitro 3D collagen-based model of skeletal muscle

Smith, AST; Mudera, V; Greensmith, L; Lewis, MP; (2011) Analysis of myotube-motoneuron interaction within an in vitro 3D collagen-based model of skeletal muscle. In: Abstracts of the Tissue and Cell Engineering Society (TCES) meeting 28-30 July 2010, University of Manchester. (pp. 19 - 19). Green open access

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Abstract

In seeking to further our understanding of skeletal muscle physiology and function in both healthy and diseased tissues, there is a strong need to develop in vitro culture systems that better represent the in vivo condition. This project is aimed at developing an innervated 3D in vitro model of skeletal muscle. It is hoped that the incorporation of primary motoneurons into a 3D model of skeletal muscle established in our lab will promote myofibre development towards an adult phenotype and improve the biomimicity of the system. Furthermore, the formation of neuromuscular junctions (NMJ) within a 3D in vitro setting should allow for testing of the effects of neuromuscular agents in culture, thereby reducing the need for in vivo experimentation. Here we present data characterising the development and maturation of this 3D co-culture system in comparison to conventional 2D cultures and discuss the implications for the future of skeletal muscle tissue culture techniques.

Type: Proceedings paper
Title: Analysis of myotube-motoneuron interaction within an in vitro 3D collagen-based model of skeletal muscle
Event: Tissue and Cell Engineering Society (TCES) meeting
Location: University of Manchester
Dates: 28 July 2010-30 July 2010
Open access status: An open access version is available from UCL Discovery
Publisher version: http://www.ecmjournal.org/journal/supplements/vol0...
Language: English
Additional information: Reproduced with kind permission from eCM journal (www.ecmjournal.org)
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci
URI: https://discovery.ucl.ac.uk/id/eprint/1348474
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