Kristiansen, M;
Menghi, F;
Hughes, R;
Hubank, M;
Ham, J;
(2011)
Global analysis of gene expression in NGF-deprived sympathetic neurons identifies molecular pathways associated with cell death.
BMC Genomics
, 12
, Article 551. 10.1186/1471-2164-12-551.
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Abstract
Developing sympathetic neurons depend on nerve growth factor (NGF) for survival and die by apoptosis after NGF withdrawal. This process requires de novo gene expression but only a small number of genes induced by NGF deprivation have been identified so far, either by a candidate gene approach or in mRNA differential display experiments. This is partly because it is difficult to obtain large numbers of sympathetic neurons for in vitro studies. Here, we describe for the first time, how advances in gene microarray technology have allowed us to investigate the expression of all known genes in sympathetic neurons cultured in the presence and absence of NGF.
| Type: | Article |
|---|---|
| Title: | Global analysis of gene expression in NGF-deprived sympathetic neurons identifies molecular pathways associated with cell death |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1186/1471-2164-12-551 |
| Publisher version: | http://dx.doi.org/10.1186/1471-2164-12-551 |
| Language: | English |
| Additional information: | © 2011 Kristiansen et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. PMCID: PMC3256215 |
| Keywords: | Animals, Binding Sites, Cell Death, Down-Regulation, Exons, Gene Expression Profiling, Nerve Growth Factor, Neurons, Promoter Regions, Genetic, Proto-Oncogene Proteins c-jun, Rats, Real-Time Polymerase Chain Reaction, Sympathetic Nervous System, Up-Regulation |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1335284 |
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