Pryor, SE;
Massa, V;
Savery, D;
Greene, NDE;
Copp, AJ;
(2012)
Convergent extension analysis in mouse whole embryo culture.
Methods in Molecular Biology
, 839
pp. 133-146.
10.1007/978-1-61779-510-7_11.
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Abstract
Mutations have been identified in a non-canonical Wnt signalling cascade (the planar cell polarity pathway) in several mouse genetic models of severe neural tube defects. In each of these models, neurulation fails to be initiated at the 3-4 somite stage, leading to an almost entirely open neural tube (termed craniorachischisis). Studies in whole embryo culture have identified a defect in the morphogenetic process of convergent extension during gastrulation, preceding the onset of neural tube closure. The principal defect is a failure of midline extension, both in the neural plate and axial mesoderm. This leads to an abnormally wide neural plate in which the elevating neural folds are too far apart to achieve closure. In this chapter, we provide details of several experimental methods that can be used to evaluate convergent extension in cultured mouse embryos. We describe analytical methods that can reveal the abnormalities that characterise neurulation-stage embryos with defective planar cell polarity signalling, in particular the loop-tail (Lp; Vangl2) mutant.
| Type: | Article |
|---|---|
| Title: | Convergent extension analysis in mouse whole embryo culture |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-61779-510-7_11 |
| Publisher version: | http://dx.doi.org/10.1007/978-1-61779-510-7_11 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Animals, Carbocyanines, Cell Polarity, Dissection, Electroporation, Embryo Culture Techniques, Embryo, Mammalian, Female, Genetic Vectors, Green Fluorescent Proteins, Mice, Mutation, Nerve Tissue Proteins, Neural Plate, Neural Tube, Neurulation, Paraffin Embedding, Phenotype, Pregnancy |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Developmental Biology and Cancer Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/1334767 |
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