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Nebulisation of Receptor-Targeted Nanocomplexes for Gene Delivery to the Airway Epithelium

Manunta, MDI; McAnulty, RJ; Tagalakis, AD; Bottoms, SE; Campbell, F; Hailes, HC; Tabor, AB; ... Hart, SL; + view all (2011) Nebulisation of Receptor-Targeted Nanocomplexes for Gene Delivery to the Airway Epithelium. PLOS ONE , 6 (10) , Article e26768. 10.1371/journal.pone.0026768. Green open access

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Abstract

Background: Gene therapy mediated by synthetic vectors may provide opportunities for new treatments for cystic fibrosis (CF) via aerosolisation. Vectors for CF must transfect the airway epithelium efficiently and not cause inflammation so they are suitable for repeated dosing. The inhaled aerosol should be deposited in the airways since the cystic fibrosis transmembrane conductance regulator gene (CFTR) is expressed predominantly in the epithelium of the submucosal glands and in the surface airway epithelium. The aim of this project was to develop an optimised aerosol delivery approach applicable to treatment of CF lung disease by gene therapy.Methodology: The vector suspension investigated in this study comprises receptor-targeting peptides, cationic liposomes and plasmid DNA that self-assemble by electrostatic interactions to form a receptor-targeted nanocomplex (RTN) of approximately 150 nm with a cationic surface charge of +50 mV. The aerodynamic properties of aerosolised nanocomplexes produced with three different nebulisers were compared by determining aerosol deposition in the different stages of a Next Generation Pharmaceutical Impactor (NGI). We also investigated the yield of intact plasmid DNA by agarose gel electrophoresis and densitometry, and transfection efficacies in vitro and in vivo.Results: RTNs nebulised with the AeroEclipse II BAN were the most effective, compared to other nebulisers tested, for gene delivery both in vitro and in vivo. The biophysical properties of the nanocomplexes were unchanged after nebulisation while the deposition of RTNs suggested a range of aerosol aerodynamic sizes between 5.5 mu m-1.4 mu m cut off (NGI stages 3-6) compatible with deposition in the central and lower airways.Conclusions: RTNs showed their ability at delivering genes via nebulisation, thus suggesting their potential applications for therapeutic interventions of cystic fibrosis and other respiratory disorders.

Type: Article
Title: Nebulisation of Receptor-Targeted Nanocomplexes for Gene Delivery to the Airway Epithelium
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0026768
Publisher version: http://dx.doi.org/10.1371/journal.pone.0026768
Language: English
Additional information: © 2011 Manunta et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The work was funded by a charity: SPARKS - The children's medical research charity. https://www.sparks.org.uk. Sparks Grant 06ICH10. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Keywords: BETA-GALACTOSIDASE ACTIVITY, GENERATION PHARMACEUTICAL IMPACTOR, PLACEBO-CONTROLLED TRIAL, CYSTIC-FIBROSIS GENE, EXPRESSION IN-VIVO, ADENOASSOCIATED VIRUS, PHYSICAL STABILITY, SYNTHETIC VECTOR, DNA COMPLEXES, DOUBLE-BLIND
UCL classification: UCL > Provost and Vice Provost Offices
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Genetics and Genomic Medicine Prog
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Pop Health Sciences > UCL GOS Institute of Child Health > ICH Infect, Imm, Infla. and Physio Med
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/1329815
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