UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

In Vivo Emergence of HIV-1 Highly Sensitive to Neutralizing Antibodies

Aasa-Chapman, MMI; Cheney, KM; Hue, S; Forsman, A; O'Farrell, S; Pellegrino, P; Williams, I; (2011) In Vivo Emergence of HIV-1 Highly Sensitive to Neutralizing Antibodies. PLOS ONE , 6 (8) , Article e23961. 10.1371/journal.pone.0023961. Green open access

[thumbnail of 1328308.pdf]
Preview
PDF
1328308.pdf

Download (1MB)

Abstract

Background: The rapid and continual viral escape from neutralizing antibodies is well documented in HIV-1 infection. Here we report in vivo emergence of viruses with heightened sensitivity to neutralizing antibodies, sometimes paralleling the development of neutralization escape.Methodology/Principal Findings: Sequential viral envs were amplified from seven HIV-1 infected men monitored from seroconversion up to 5 years after infection. Env-recombinant infectious molecular clones were generated and tested for coreceptor use, macrophage tropism and neutralization sensitivity to homologous and heterologous serum, soluble CD4 and monoclonal antibodies IgG1b12, 2G12 and 17b. We found that HIV-1 evolves sensitivity to contemporaneous neutralizing antibodies during infection. Neutralization sensitive viruses grow out even when potent autologous neutralizing antibodies are present in patient serum. Increased sensitivity to neutralization was associated with susceptibility of the CD4 binding site or epitopes induced after CD4 binding, and mediated by complex envelope determinants including V3 and V4 residues. The development of neutralization sensitive viruses occurred without clinical progression, coreceptor switch or change in tropism for primary macrophages.Conclusions: We propose that an interplay of selective forces for greater virus replication efficiency without the need to resist neutralizing antibodies in a compartment protected from immune surveillance may explain the temporal course described here for the in vivo emergence of HIV-1 isolates with high sensitivity to neutralizing antibodies.

Type: Article
Title: In Vivo Emergence of HIV-1 Highly Sensitive to Neutralizing Antibodies
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0023961
Publisher version: http://dx.doi.org/10.1371/journal.pone.0023961
Language: English
Additional information: © 2011 Aasa-Chapman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. This work was supported by the Wellcome Trust, the MRC-UK and core funding from the Edward Jenner Institute for Vaccine Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Keywords: HUMAN-IMMUNODEFICIENCY-VIRUS, HUMAN MONOCLONAL-ANTIBODY, ENVELOPE GLYCOPROTEINS, ENV CLONES, CELL-LINE, TYPE-1, INFECTION, GP120, ESCAPE, EVOLUTION
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute for Global Health > Infection and Population Health
URI: https://discovery.ucl.ac.uk/id/eprint/1328308
Downloads since deposit
128Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item