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Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)

Warburton, PJ; Allan, E; Hunter, S; Ward, J; Booth, V; Wade, WG; Mullany, P; (2011) Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA). FEMS Microbiology Ecology , 78 (2) 349 - 354. 10.1111/j.1574-6941.2011.01166.x. Green open access

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Abstract

The human oral cavity is host to a complex microbial community estimated to comprise > 700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.

Type: Article
Title: Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA)
Open access status: An open access version is available from UCL Discovery
DOI: 10.1111/j.1574-6941.2011.01166.x
Publisher version: http://dx.doi.org/10.1111/j.1574-6941.2011.01166.x
Language: English
Additional information: Re-use of this article is permitted in accordance with the Term and Conditions set out at http://wileyonlinelibrary. com/onlineopen#OnlineOpen-Terms
Keywords: oral cavity, plasmid, TRACA, dental plaque, metagenome, STREPTOCOCCUS-MUTANS, NUCLEOTIDE-SEQUENCE, SUBGINGIVAL PLAQUE, CRYPTIC PLASMID, DIVERSITY, RESISTANCE, STRAIN, SITES
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Microbial Diseases
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/1315138
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