Shillingford, Daisy;
Radek, Andreas;
Kupitz, Andrea;
Thomas, Rebecca;
Connor, Christopher;
Adams, Stuart Paul;
(2025)
Validation of a New High-Throughput Cell Separation Method for Downstream Molecular Applications.
International Journal of Molecular Sciences
, 26
(14)
, Article 6747. 10.3390/ijms26146747.
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Abstract
The development of new cell separation technologies has continued as the demand for sorted cell populations for molecular testing increases. The goal is to increase through-put potential and reduce the manual handling of samples required whilst ensuring that cells are sorted efficiently with high purity. Herein, we review two affinity-based methods utilising magnetic beads to isolate cells: one is currently used within a clinical laboratory as standard of care and the other is a newly developed larger platform using the same principle. Cells were sorted simultaneously on both platforms and assessments were made of the purity, cell recovery, and hands-on time, indicating that the new larger platform is sufficient for use in a clinical laboratory as it not only increased cell sorting capacity and reduced manual processing but was also able to isolate cells with sufficient purity levels for downstream molecular testing.
| Type: | Article |
|---|---|
| Title: | Validation of a New High-Throughput Cell Separation Method for Downstream Molecular Applications |
| Location: | Switzerland |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/ijms26146747 |
| Publisher version: | https://doi.org/10.3390/ijms26146747 |
| Language: | English |
| Additional information: | Copyright © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | cell sorting, T cells, B cells, granulocytes, chimerism |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10215484 |
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