Park, Seungbin;
Lipton, Megan;
Sun, Yujiao J;
Dadarlat, Maria;
(2024)
Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging.
STAR Protocols
, 5
(2)
, Article 103027. 10.1016/j.xpro.2024.103027.
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Abstract
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al.<sup>1</sup>
| Type: | Article |
|---|---|
| Title: | Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.xpro.2024.103027 |
| Publisher version: | https://doi.org/10.1016/j.xpro.2024.103027 |
| Language: | English |
| Additional information: | © 2024 The Authors. Published by Elsevier Inc. under a Creative Commons license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10214412 |
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