Carroll, Melissa S;
Giacca, Mauro;
(2023)
CRISPR activation and interference as investigative tools in the cardiovascular system.
The International Journal of Biochemistry & Cell Biology
, 155
, Article 106348. 10.1016/j.biocel.2022.106348.
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Abstract
CRISPR activation and interference (CRISPRa/i) technology offers the unprecedented possibility of achieving regulated gene expression both in vitro and in vivo. The DNA pairing specificity of a nuclease dead Cas9 (dCas9) is exploited to precisely target a transcriptional activator or repressor in proximity to a gene promoter. This permits both the study of phenotypes arising from gene modulation for investigative purposes, and the development of potential therapeutics. As with virtually all other organ systems, the cardiovascular system can deeply benefit from a broader utilisation of CRISPRa/i. However, application of this technology is still in its infancy. Significant areas for improvement include the identification of novel and more effective transcriptional regulators that can be docked to dCas9, and the development of more efficient methods for their delivery and expression in vivo.
| Type: | Article |
|---|---|
| Title: | CRISPR activation and interference as investigative tools in the cardiovascular system |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.biocel.2022.106348 |
| Publisher version: | https://doi.org/10.1016/j.biocel.2022.106348 |
| Language: | English |
| Additional information: | This work is licensed under a Creative Commons License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Cell Biology, CRISPR, DCas9, Transcriptional regulation, Cardiovascular, SEQUENCE-SPECIFIC CONTROL, IN-VIVO, DNA METHYLATION, GENE ACTIVATION, TRANSCRIPTION, DISEASE |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10212757 |
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