Froghi, Saied;
(2025)
Effect of Histotripsy in decellularization of liver tissue &
Histotripsy-assisted stem cell derived 3D-hepatocyte
implantation for liver regeneration.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
Chronic liver diseases, including cirrhosis and hepatocellular carcinoma, remain significant global health challenges due to their high morbidity and limited therapeutic options. This thesis investigates the potential of histotripsy, a non-invasive focused ultrasound technology, in two innovative applications: decellularization of liver tissue and facilitation of stem cell-derived 3D-hepatocyte transplantation for liver regeneration. The first segment explores histotripsy’s efficacy in generating acellular liver scaffolds, emphasizing its mechanical precision in preserving vascular structures while minimizing collateral damage. Experimental methods, including histological assessments and 3D tissue reconstructions, demonstrate the feasibility of histotripsy for creating biocompatible scaffolds, paving the way for advanced tissue engineering applications. The second segment evaluates histotripsy-assisted implantation of stem cell-derived 3D hepatocyte spheroids in rodent models, aiming to overcome challenges in cell delivery, engraftment, and survival. Key findings include improved cell viability, enhanced tissue integration, and evidence of functional liver regeneration post-implantation. The thesis highlights histotripsy’s potential to synergize with regenerative therapies, offering a transformative approach to address organ shortages and advance treatment strategies for end-stage liver diseases. These findings underscore histotripsy’s dual utility in liver tissue engineering and regenerative medicine, offering a foundation for future translational research and clinical applications.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Effect of Histotripsy in decellularization of liver tissue & Histotripsy-assisted stem cell derived 3D-hepatocyte implantation for liver regeneration |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Copyright © The Author 2025. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10211508 |
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