Bhaskar, Haresh;
Gidden, Zoe;
Virdi, Gurvir;
Kleinjan, Dirk-Jan;
Rosser, Susan J;
Gandhi, Sonia;
Regan, Lynne;
(2025)
Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT.
Protein Science
, 34
(2)
, Article e70008. 10.1002/pro.70008.
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Abstract
Super-resolution microscopy has revolutionized biological imaging, enabling the visualization of structures at the nanometer length scale. Its application in live cells, however, has remained challenging. To address this, we adapted LIVE-PAINT, an approach we established in yeast, for application in live mammalian cells. Using the 101A/101B coiled-coil peptide pair as a peptide-based targeting system, we successfully demonstrate the super-resolution imaging of two distinct proteins in mammalian cells, one localized in the nucleus, and the second in the cytoplasm. This study highlights the versatility of LIVE-PAINT, suggesting its potential for live-cell super-resolution imaging across a range of protein targets in mammalian cells. We name the mammalian cell version of our original method mLIVE-PAINT.
| Type: | Article |
|---|---|
| Title: | Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/pro.70008 |
| Publisher version: | https://doi.org/10.1002/pro.70008 |
| Language: | English |
| Additional information: | © 2025 The Author(s). Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, dynamics, mitochondria, nucleus, peptide-peptide interactions, single-molecule, super-resolution microscopy, MICROSCOPY, FLUORESCENCE, EXPRESSION, RESOLUTION |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Clinical and Movement Neurosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10204457 |
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