Tosolini, Andrew P;
Abatecola, Federico;
Negro, Samuele;
Sleigh, James N;
Schiavo, Giampietro;
(2024)
The node of Ranvier influences the in vivo axonal transport of mitochondria and signalling endosomes.
iScience
, Article 111158. 10.1016/j.isci.2024.111158.
(In press).
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Abstract
Efficient long-range axonal transport is essential for maintaining neuronal function, and perturbations in this process underlie severe neurological diseases. Nodes of Ranvier (NoR) are short, specialised unmyelinated axonal domains with a unique molecular and structural composition. Currently, it remains unresolved how the distinct molecular structures of the NoR impact axonal transport dynamics. Using intravital time-lapse microscopy of sciatic nerves in live, anaesthetised mice, we reveal 1) similar morphologies of the NoR in fast and slow motor axons, 2) signalling endosomes and mitochondria accumulate specifically at the distal node, and 3) unique axonal transport profiles of signalling endosomes and mitochondria transiting through the NoR. Collectively, these findings provide important insights into the fundamental physiology of peripheral nerve axons, motor neuron subtypes and diverse organelle dynamics at the NoR. Furthermore, this work has relevance for several pathologies affecting peripheral nerves and the NoR.
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