Temisak, S;
Thangsunan, P;
Boonnil, J;
Yenchum, W;
Hongthong, K;
Oss Boll, H;
Yata, T;
... Morris, P; + view all
(2021)
Accurate determination of meat mass fractions using DNA measurements for quantifying meat adulteration by digital PCR.
International Journal of Food Science and Technology
, 56
(12)
pp. 6345-6358.
10.1111/ijfs.15375.
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Abstract
The alarming problem of meat adulteration emphasises the demand for accessible analytical approaches for food regulatory agencies to detect and, specially, to measure altered meat fractions. This study proposes a novel cross-species triplex droplet digital polymerase chain reaction (ddPCR) assay to simultaneously identify and quantify the ratios of pork/beef meat fractions from a total DNA content, including processed and autoclaved meat, without requiring a standard, achieving high sensitivity with a limit of quantification estimated at 0.1% (w/w) and a limit of detection down to 0.01% (w/w). A single copy nuclear gene, β-actin, was employed as a target, accompanied with myostatin gene as a cross-species target to quantify the meat background. The duplex assay provided a simultaneous quantification of pork and myostatin, whereas the triplex assay was able to detect pork, beef and myostatin with a decrease of technical error, cost and time.
| Type: | Article |
|---|---|
| Title: | Accurate determination of meat mass fractions using DNA measurements for quantifying meat adulteration by digital PCR |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1111/ijfs.15375 |
| Publisher version: | http://dx.doi.org/10.1111/ijfs.15375 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | DNA measurements, droplet digital PCR, food mislabelling, mass fractions, meat quantification, pork/beef adulteration |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > UCL BEAMS UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10194383 |
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