Yardimci, Hasan;
Loveland, Anna B;
Habuchi, Satoshi;
van Oijen, Antoine M;
Walter, Johannes C;
(2010)
Uncoupling of Sister Replisomes during Eukaryotic DNA Replication.
Molecular Cell
, 40
(5)
pp. 834-840.
10.1016/j.molcel.2010.11.027.
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Abstract
The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that they travel away from one another and thus function independently. Alternatively, sister replisomes may form a stationary, functional unit that draws parental DNA toward itself. If this "double replisome" model is correct, a constrained DNA molecule should not undergo replication. To test this prediction, lambda DNA was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication involves autonomously functioning replisomes.
| Type: | Article |
|---|---|
| Title: | Uncoupling of Sister Replisomes during Eukaryotic DNA Replication |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.molcel.2010.11.027 |
| Publisher version: | http://dx.doi.org/10.1016/j.molcel.2010.11.027 |
| Language: | English |
| Additional information: | Articles published under an Elsevier user license are protected by copyright and may be used for non-commercial purposes. Users may access, download, copy, translate, text mine and data mine the articles. https://www.elsevier.com/about/policies-and-standards/open-access-licenses/elsevier-user |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Cell Biology, XENOPUS EGG EXTRACT, VIRAL ORIGIN, COMPLEX, FORK, ASSOCIATION, INITIATION, CHROMATIN, CDC45, MCM, REPLICONS |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10191058 |
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