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Single-molecule imaging reveals control of parental histone recycling by free histones during DNA replication

Gruszka, DT; Xie, S; Kimura, H; Yardimci, H; (2020) Single-molecule imaging reveals control of parental histone recycling by free histones during DNA replication. Science Advances , 6 (38) , Article eabc0330. 10.1126/sciadv.abc0330. Green open access

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Abstract

During replication, nucleosomes are disrupted ahead of the replication fork, followed by their reassembly on daughter strands from the pool of recycled parental and new histones. However, because no previous studies have managed to capture the moment that replication forks encounter nucleosomes, the mechanism of recycling has remained unclear. Here, through real-time single-molecule visualization of replication fork progression in Xenopus egg extracts, we determine explicitly the outcome of fork collisions with nucleosomes. Most of the parental histones are evicted from the DNA, with histone recycling, nucleosome sliding, and replication fork stalling also occurring but at lower frequencies. Critically, we find that local histone recycling becomes dominant upon depletion of endogenous histones from extracts, revealing that free histone concentration is a key modulator of parental histone dynamics at the replication fork. The mechanistic details revealed by these studies have major implications for our understanding of epigenetic inheritance.

Type: Article
Title: Single-molecule imaging reveals control of parental histone recycling by free histones during DNA replication
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1126/sciadv.abc0330
Publisher version: http://dx.doi.org/10.1126/sciadv.abc0330
Language: English
Additional information: Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Keywords: Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, PHASE-SEPARATION, CHROMATIN, DYNAMICS, FORK
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10191051
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